A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics
Sign Up to like & getrecommendations! Published in 2021 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.1c00181
Abstract: Rapid, point-of-care (POC) diagnostics are essential to mitigate the impacts of current (and future) epidemics; however, current methods for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) require complicated laboratory tests that are generally conducted… read more here.
Keywords: system; crispr; rna dna; sars cov ... See more keywords
Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
Sign Up to like & getrecommendations! Published in 2021 at "Nature Methods"
DOI: 10.1038/s41592-021-01124-4
Abstract: Competitive coevolution between microbes and viruses has led to the diversification of CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic terabase datasets, we identified two compact families (775 to 803 amino acids (aa)) of… read more here.
Keywords: rna base; rna editing; cas13; programmable rna ... See more keywords
Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118260119
Abstract: Significance Thermostable Cas13 orthologues will expand and unlock the power of CRISPR systems for synthetic biology and biotechnology applications. We identified and characterized a thermostable Cas13 orthologue from Thermoclostridium caenicola (TccCas13a), which exhibits robust cis… read more here.
Keywords: cas13; thermostable cas13; one pot; characterization thermostable ... See more keywords
RNA-mediated CRISPR-Cas13 inhibition through crRNA structural mimicry.
Sign Up to like & getrecommendations! Published in 2025 at "Science"
DOI: 10.1126/science.adr3656
Abstract: To circumvent CRISPR-Cas immunity, phages express anti-CRISPR factors that inhibit the expression or activities of Cas proteins. Whereas most anti-CRISPRs described to date are proteins, recently described small RNAs called RNA anti-CRISPRs (rAcrs) have sequence… read more here.
Keywords: rna; cas13; crispr cas13; structural mimicry ... See more keywords
Systemic evaluation of various CRISPR/Cas13 orthologs for knockdown of targeted transcripts in plants
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DOI: 10.1186/s13059-024-03448-8
Abstract: CRISPR/Cas13 system, recognized for its compact size and specificity in targeting RNA, is currently employed for RNA degradation. However, the potential of various CRISPR/Cas13 subtypes, particularly concerning the knockdown of endogenous transcripts, remains to be… read more here.
Keywords: cas13; cas13 orthologs; crispr cas13; various crispr ... See more keywords
Programmable System of Cas13-Mediated RNA Modification and Its Biological and Biomedical Applications
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2021.677587
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13 has drawn broad interest to control gene expression and cell fate at the RNA level in general. Apart from RNA interference mediated by its endonuclease activity, the nuclease-deactivated… read more here.
Keywords: rna; biology; system cas13; cas13 ... See more keywords
CRISPR-Cas13-Mediated Knockdown of Regulator of G-Protein Signaling 8 (RGS8) Does Not Affect Purkinje Cell Dendritic Development
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.854273
Abstract: CRISPR-Cas13 technology is rapidly evolving as it is a very specific tool for RNA editing and interference. Since there are no significant off-target effects via the Cas13-mediated method, it is a promising tool for studying… read more here.
Keywords: regulator protein; cas13; crispr cas13; purkinje ... See more keywords
Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.866820
Abstract: Ribonucleic acid (RNA) and proteins play critical roles in gene expression and regulation. The relevant study increases the understanding of various life processes and contributes to the diagnosis and treatment of different diseases. RNA imaging… read more here.
Keywords: cas13; rna; rna protein; protein interactions ... See more keywords
The Bacterial Enzyme Cas13 Interferes with Neurite Outgrowth from Cultured Cortical Neurons
Sign Up to like & getrecommendations! Published in 2021 at "Toxins"
DOI: 10.3390/toxins13040262
Abstract: The CRISPR-Cas13 system based on a bacterial enzyme has been explored as a powerful new method for RNA manipulation. Due to the high efficiency and specificity of RNA editing/interference achieved by this system, it is… read more here.
Keywords: cas13; bacterial enzyme; cortical neurons; crispr cas13 ... See more keywords