A CRISPR-Cas13a system for efficient and specific therapeutic targeting of mutant KRAS for pancreatic cancer treatment.
Sign Up to like & getrecommendations! Published in 2018 at "Cancer letters"
DOI: 10.1016/j.canlet.2018.05.042
Abstract: Mutant KRAS is a known driver oncogene in pancreatic cancer. However, this protein remains an "undruggable" therapeutic target. Inhibiting mutated KRAS expression at the mRNA level is a potentially effective strategy. Recently, a novel CRISPR-Cas… read more here.
Keywords: crispr cas13a; mutant kras; cas13a; cas13a system ... See more keywords
A novel rapid visual detection assay for Toxoplasma gondii combining recombinase-aided amplification and lateral flow dipstick coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD)
Sign Up to like & getrecommendations! Published in 2022 at "Parasite"
DOI: 10.1051/parasite/2022021
Abstract: Toxoplasmosis, a parasitic disease resulting from Toxoplasma gondii infection, remains prevalent worldwide, and causes great harm to immunodepressed patients, pregnant women and newborns. Although various molecular approaches to detect T. gondii infection are available, they… read more here.
Keywords: raa cas13a; cas13a lfd; cas13a; gondii ... See more keywords
Rapid detection of insect pest with CRISPR‐Cas13a‐Based Lateral Flow Strip: Using Locusta migratoria manilensis
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Applied Entomology"
DOI: 10.1111/jen.13121
Abstract: The Clustered Regularly Interspaced Short Palindromic Repeats‐associated (CRISPR‐Cas) system has been used as a powerful diagnostic tool because of its trans‐cleavage ability to accurately recognize and cleave specific nucleic acid targets. However, its application in… read more here.
Keywords: system; cas13a; migratoria manilensis; crispr cas13a ... See more keywords
Hierarchical self-uncloaking CRISPR-Cas13a–customized RNA nanococoons for spatial-controlled genome editing and precise cancer therapy
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abn7382
Abstract: CRISPR-Cas13a holds enormous potential for developing precise RNA editing. However, spatial manipulation of CRISPR-Cas13a activity remains a daunting challenge for elaborately regulating localized RNase function. Here, we designed hierarchical self-uncloaking CRISPR-Cas13a–customized RNA nanococoons (RNCOs-D), featuring… read more here.
Keywords: cas13a; therapy; crispr cas13a; hierarchical self ... See more keywords
Nucleic acid detection with CRISPR-Cas13a/C2c2
Sign Up to like & getrecommendations! Published in 2017 at "Science"
DOI: 10.1126/science.aam9321
Abstract: Sensitive and specific CRISPR diagnostics Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a… read more here.
Keywords: detection; crispr; cas13a; dna ... See more keywords
Differentiation of Classical Swine Fever Virus Virulent and Vaccine Strains by CRISPR/Cas13a
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00891-22
Abstract: Classical swine fever (CSF) is a World Organisation for Animal Health (formerly the Office International des Epizooties [OIE]) notifiable terrestrial and aquatic animal disease, causing great economic losses to the swine industry worldwide during the… read more here.
Keywords: cas13a; crispr cas13a; virus; swine fever ... See more keywords
Rapid Detection of Tomato Spotted Wilt Virus With Cas13a in Tomato and Frankliniella occidentalis
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.745173
Abstract: As one of the top 10 plant viruses, the severity of losses to crop productivity caused by the tomato spotted wilt virus (TSWV) has resulted in an urgent need to develop a more sensitive and… read more here.
Keywords: detection system; detection; cas13a; tomato ... See more keywords
CRISPR-Cas13a Based Visual Detection Assays for Feline Calicivirus Circulating in Southwest China
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.913780
Abstract: Feline calicivirus (FCV) is a well-known causative pathogen for upper respiratory infection in cats. Its high genetic variability challenges existing molecular diagnostic methods in clinical settings. Thus, we developed two sensitive and visual assays for… read more here.
Keywords: cas13a; feline calicivirus; crispr cas13a; detection ... See more keywords
An Ultrasensitive PCR-Based CRISPR-Cas13a Method for the Detection of Helicobacter pylori
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Personalized Medicine"
DOI: 10.3390/jpm12122082
Abstract: The rapid and simple detection of Helicobacter pylori (H. pylori) is essential for its clinical eradication. Although various methods for detecting H. pylori have been well established, such as endoscopy in combination with histology or… read more here.
Keywords: detection helicobacter; cas13a; crispr; detection ... See more keywords
Hierarchically tumor-activated nanoCRISPR-Cas13a facilitates efficient microRNA disruption for multi-pathway-mediated tumor suppression
Sign Up to like & getrecommendations! Published in 2023 at "Theranostics"
DOI: 10.7150/thno.81776
Abstract: Rationale: CRISPR-Cas13a is an efficient tool for robust RNA knockdown with lower off-target effect, which may be a potentially powerful and safe tool for cancer gene therapy. However, therapeutic effect of current cancer gene therapy… read more here.
Keywords: cas13a; tumor; multi pathway; suppression ... See more keywords