Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d
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DOI: 10.1016/j.cell.2018.09.001
Abstract: CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and… read more here.
Keywords: structural basis; rna guided; ribonuclease; rna ... See more keywords
Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10507-3
Abstract: Cas13d, the type VI-D CRISPR-Cas effector, is an RNA-guided ribonuclease that has been repurposed to edit RNA in a programmable manner. Here we report the detailed structural and functional analysis of the uncultured Ruminococcus sp.… read more here.
Keywords: rna; crrna; cas13d; target cleavage ... See more keywords
Efficient nanoparticle-based CRISPR-Cas13d induced mRNA disruption of an eye pigmentation gene in the white-backed planthopper, Sogatella furcifera.
Sign Up to like & getrecommendations! Published in 2023 at "Insect science"
DOI: 10.1111/1744-7917.13203
Abstract: The discovery of the clustered regularly interspaced short palindromic repeat (CRISPR) system has driven gene manipulation technology to a new era with applications reported in organisms that span the tree of life. The utility of… read more here.
Keywords: gene; crispr; cas13d; pigmentation gene ... See more keywords
Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs
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DOI: 10.1186/s13059-021-02263-9
Abstract: Short hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool… read more here.
Keywords: functional circrnas; optimized rna; rna targeting; crispr cas13d ... See more keywords
Shotgun knockdown of RNA by CRISPR-Cas13d in fission yeast.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cell science"
DOI: 10.1242/jcs.260769
Abstract: The CRISPR-Cas13d system has a single small effector protein that targets RNA and does not require the presence of a protospacer flanking site in the targeted transcript. These features make CRISPR-Cas13d an attractive system for… read more here.
Keywords: cas13d system; crispr cas13d; fission yeast; cas13d ... See more keywords
Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.960192
Abstract: Type VI CRISPR effector Cas13d from Ruminococcus flavefaciens XPD3002 (RfxCas13d) is an RNA-guided RNA endonuclease. RfxCas13d has been harnessed to knockdown gene expression with high specificity in various systems including mammalian cells. While inducible knockdown… read more here.
Keywords: cas13d; efficient reversible; lentivirus vector; vector ... See more keywords