Repetitive Loop Formation by the CRISPR-Cas3 Helicase
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.430
Abstract: E. coli maintain CRISPR-Cas adaptive immune systems to protect the cell against invading genetic elements. Immunity relies on the RNA guided surveillance complex Cascade (CRISPR-associated complex for antiviral defense) and the trans-acting Cas3 protein with… read more here.
Keywords: loop formation; crispr; repetitive loop; cas3 ... See more keywords
Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.03.031
Abstract: CRISPR-Cas provides RNA-guided adaptive immunity against invading genetic elements. Interference in type I systems relies on the RNA-guided Cascade complex for target DNA recognition and the Cas3 helicase/nuclease protein for target degradation. Even though the… read more here.
Keywords: dna; repetitive dna; dna reeling; target ... See more keywords
Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)
Sign Up to like & getrecommendations! Published in 2024 at "Communications Biology"
DOI: 10.1038/s42003-024-06537-3
Abstract: R-loop-triggered collateral single-stranded DNA (ssDNA) nuclease activity within Class 1 Type I CRISPR-Cas systems holds immense potential for nucleic acid detection. However, the hyperactive ssDNase activity of Cas3 introduces unwanted noise and false-positive results. In… read more here.
Keywords: cas3; hpv; crispr cas3; type crispr ... See more keywords
Cas3-stimulated runaway replication of modified ColE1 plasmids in Escherichia coli is temperature dependent.
Sign Up to like & getrecommendations! Published in 2019 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnz106
Abstract: The clustered regularly interspersed short palindromic repeats (CRISPR)-Cas system constitutes an adaptive immunity system of prokaryotes against mobile genetic elements using a CRISPR RNA (crRNA)-mediated interference mechanism. In Type I CRISPR-Cas systems, crRNA guided by… read more here.
Keywords: plasmid replication; cas3 stimulated; crispr cas; runaway replication ... See more keywords
Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4
Sign Up to like & getrecommendations! Published in 2025 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02572-24
Abstract: ABSTRACT Bacterial plasmid encoding antibiotic resistance could be eradicated by various CRISPR systems, such as CRISPR-Cas9, Cas12f1, and Cas3. However, the efficacy of these gene editing tools against bacterial resistance has not been systematically assessed… read more here.
Keywords: cas3; kpc imp; resistance; cas12f1 ... See more keywords
A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22062848
Abstract: Cas3 is a ssDNA-targeting nuclease-helicase essential for class 1 prokaryotic CRISPR immunity systems, which has been utilized for genome editing in human cells. Cas3-DNA crystal structures show that ssDNA follows a pathway from helicase domains… read more here.
Keywords: cas3 nuclease; crispr; temperature; nuclease ... See more keywords
Anion Inhibition Studies of the β-Class Carbonic Anhydrase CAS3 from the Filamentous Ascomycete Sordaria macrospora
Sign Up to like & getrecommendations! Published in 2020 at "Metabolites"
DOI: 10.3390/metabo10030093
Abstract: CAS3 is a newly cloned cytosolic β-class carbonic anhydrase (CA, EC 4.2.1.1) from the filamentous ascomycete Sordaria macrospora. This enzyme has a high catalytic activity for the physiological CO2 hydration reaction and herein, we report… read more here.
Keywords: carbonic anhydrase; inhibition; ascomycete sordaria; class carbonic ... See more keywords