LAUSR

About LAUSR
Donate
Contact Us

Follow us:

You are not signed in:

Sign Up!


   Articles with "cas3" as a keyword



Photo from wikipedia

Repetitive Loop Formation by the CRISPR-Cas3 Helicase

Sign Up to like & get
recommendations! Published in 2017 at "Biophysical Journal"

DOI: 10.1016/j.bpj.2016.11.430

Abstract: E. coli maintain CRISPR-Cas adaptive immune systems to protect the cell against invading genetic elements. Immunity relies on the RNA guided surveillance complex Cascade (CRISPR-associated complex for antiviral defense) and the trans-acting Cas3 protein with… read more here.

Keywords: loop formation; crispr; repetitive loop; cas3 ... See more keywords
Photo by nci from unsplash

Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps.

Sign Up to like & get
recommendations! Published in 2018 at "Molecular cell"

DOI: 10.1016/j.molcel.2018.03.031

Abstract: CRISPR-Cas provides RNA-guided adaptive immunity against invading genetic elements. Interference in type I systems relies on the RNA-guided Cascade complex for target DNA recognition and the Cas3 helicase/nuclease protein for target degradation. Even though the… read more here.

Keywords: dna; repetitive dna; dna reeling; target ... See more keywords
Photo by anniespratt from unsplash

Cas3-stimulated runaway replication of modified ColE1 plasmids in Escherichia coli is temperature dependent.

Sign Up to like & get
recommendations! Published in 2019 at "FEMS microbiology letters"

DOI: 10.1093/femsle/fnz106

Abstract: The clustered regularly interspersed short palindromic repeats (CRISPR)-Cas system constitutes an adaptive immunity system of prokaryotes against mobile genetic elements using a CRISPR RNA (crRNA)-mediated interference mechanism. In Type I CRISPR-Cas systems, crRNA guided by… read more here.

Keywords: plasmid replication; cas3 stimulated; crispr cas; runaway replication ... See more keywords
Photo from wikipedia

A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity

Sign Up to like & get
recommendations! Published in 2021 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms22062848

Abstract: Cas3 is a ssDNA-targeting nuclease-helicase essential for class 1 prokaryotic CRISPR immunity systems, which has been utilized for genome editing in human cells. Cas3-DNA crystal structures show that ssDNA follows a pathway from helicase domains… read more here.

Keywords: cas3 nuclease; crispr; temperature; nuclease ... See more keywords
Photo by fhavlik from unsplash

Anion Inhibition Studies of the β-Class Carbonic Anhydrase CAS3 from the Filamentous Ascomycete Sordaria macrospora

Sign Up to like & get
recommendations! Published in 2020 at "Metabolites"

DOI: 10.3390/metabo10030093

Abstract: CAS3 is a newly cloned cytosolic β-class carbonic anhydrase (CA, EC 4.2.1.1) from the filamentous ascomycete Sordaria macrospora. This enzyme has a high catalytic activity for the physiological CO2 hydration reaction and herein, we report… read more here.

Keywords: carbonic anhydrase; inhibition; ascomycete sordaria; class carbonic ... See more keywords