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   Articles with "cas9" as a keyword



Effective Genome Editing Using CRISPR‐Cas9 Nanoflowers

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recommendations! Published in 2022 at "Advanced Healthcare Materials"

DOI: 10.1002/adhm.202102365

Abstract: CRISPR‐Cas9 as a powerful gene‐editing tool has tremendous potential for the treatment of genetic diseases. Herein, a new mesoporous nanoflower (NF)‐like delivery nanoplatform termed Cas9‐NF is reported by crosslinking Cas9 and polymeric micelles that enables… read more here.

Keywords: genome editing; crispr cas9; effective genome; editing using ... See more keywords

Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.

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recommendations! Published in 2019 at "Advanced materials"

DOI: 10.1002/adma.201902575

Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.

Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords

A DNA/Upconversion Nanoparticle Complex Enables Controlled Co‐Delivery of CRISPR‐Cas9 and Photodynamic Agents for Synergistic Cancer Therapy

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recommendations! Published in 2024 at "Advanced Materials"

DOI: 10.1002/adma.202309534

Abstract: Photodynamic therapy (PDT) depends on the light‐irradiated exciting of photosensitizer (PS) to generate reactive oxygen species (ROS), which faces challenges and limitations in hypoxia and antioxidant response of cancer cells, and limited tissue‐penetration of light.… read more here.

Keywords: cancer; cas9; dna upconversion; enables controlled ... See more keywords

CRISPR/Cas9 mediated validation of spermatogenesis-related gene, tssk2 as a component of genetic pest management of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae).

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recommendations! Published in 2024 at "Archives of insect biochemistry and physiology"

DOI: 10.1002/arch.22121

Abstract: Invasive insect pests, currently, pose a serious economic threat to several staple crops all over the world, one such being the fall armyworm, Spodoptera frugiperda. It was first observed in Africa since 2016, outside of… read more here.

Keywords: cas9; spodoptera frugiperda; armyworm spodoptera; management ... See more keywords
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Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On‐ and Off‐Target Damage

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recommendations! Published in 2020 at "BioEssays"

DOI: 10.1002/bies.202000047

Abstract: Large deletions and genomic re‐arrangements are increasingly recognized as common products of double‐strand break repair at Clustered Regularly Interspaced, Short Palindromic Repeats ‐ CRISPR associated protein 9 (CRISPR/Cas9) on‐target sites. Together with well‐known off‐target editing… read more here.

Keywords: crispr cas9; cas9 target; cas9 editing; target ... See more keywords

Resolving the Trade‐Off Between Toxicity and Efficiency of CRISPR‐Cas9 System for Genome Editing Within Escherichia coli

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recommendations! Published in 2025 at "Biotechnology Journal"

DOI: 10.1002/biot.70010

Abstract: Efficient gene editing of Escherichia coli BL21 (DE3) holds significant practical value as a host for heterologous protein expression. Recently reported CRISPR‐Cas9 editing systems for this strain exhibit a trade‐off between efficiency and toxicity. In… read more here.

Keywords: trade; system; efficiency; cas9 ... See more keywords

Modulation of CRISPR‐Cas9 Cleavage with an Oligo‐Ribonucleoprotein Design

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recommendations! Published in 2025 at "Chembiochem"

DOI: 10.1002/cbic.202400821

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) associated protein Cas9 system has been widely used for genome editing. However, the editing or cleavage specificity of CRISPR Cas9 remains a major concern due to the off‐target… read more here.

Keywords: cleavage; cas9; cas9 cleavage; modification ... See more keywords
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Genome Editing in Mice Using CRISPR/Cas9 Technology

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recommendations! Published in 2018 at "Current Protocols in Cell Biology"

DOI: 10.1002/cpcb.57

Abstract: CRISPR/Cas9 technology has revolutionized genome editing in mice, allowing for simple and rapid development of knockouts and knockins. CRISPR relies on small guide RNAs that direct the RNA‐guided nuclease Cas9 to a designated genomic site… read more here.

Keywords: genome editing; crispr cas9; cas9 technology; cas9 ... See more keywords
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Efficient CRISPR/Cas9‐Mediated Mutagenesis in Primary Murine T Lymphocytes

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recommendations! Published in 2019 at "Current Protocols in Immunology"

DOI: 10.1002/cpim.62

Abstract: The ability to alter gene expression directly in T lymphocytes has provided a powerful tool for understanding T cell biology, signaling, and function. Manipulation of T cell clones and primary T cells has been accomplished… read more here.

Keywords: mediated mutagenesis; mutagenesis primary; crispr cas9; cas9 mediated ... See more keywords
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Chemical Modification and Transformation Strategies of Guide RNAs in CRISPR-Cas9 Gene Editing Systems.

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recommendations! Published in 2021 at "ChemPlusChem"

DOI: 10.1002/cplu.202000785

Abstract: The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated protein 9) is a most powerful tool and has been widely used in gene editing and gene regulation since its discovery. However, wild-type CRISPR-Cas9 suffers from… read more here.

Keywords: guide rnas; gene editing; crispr cas9; gene ... See more keywords

CRISPR/Cas9 effectively generate chromosome structural variations in rice protoplasts

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recommendations! Published in 2024 at "Crop Science"

DOI: 10.1002/csc2.21334

Abstract: Chromosome structural variations (SVs), such as deletion, duplication, inversion, and translocation, are important contributors to genetic diversification and crop improvement. Using genome editing tools such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated nuclease (Cas9),… read more here.

Keywords: structural variations; rice; chromosome structural; rice protoplasts ... See more keywords