Effective Genome Editing Using CRISPR‐Cas9 Nanoflowers
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202102365
Abstract: CRISPR‐Cas9 as a powerful gene‐editing tool has tremendous potential for the treatment of genetic diseases. Herein, a new mesoporous nanoflower (NF)‐like delivery nanoplatform termed Cas9‐NF is reported by crosslinking Cas9 and polymeric micelles that enables… read more here.
Keywords: genome editing; crispr cas9; effective genome; editing using ... See more keywords
Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
Sign Up to like & getrecommendations! Published in 2019 at "Advanced materials"
DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On‐ and Off‐Target Damage
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DOI: 10.1002/bies.202000047
Abstract: Large deletions and genomic re‐arrangements are increasingly recognized as common products of double‐strand break repair at Clustered Regularly Interspaced, Short Palindromic Repeats ‐ CRISPR associated protein 9 (CRISPR/Cas9) on‐target sites. Together with well‐known off‐target editing… read more here.
Keywords: crispr cas9; cas9 target; cas9 editing; target ... See more keywords
Genome Editing in Mice Using CRISPR/Cas9 Technology
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DOI: 10.1002/cpcb.57
Abstract: CRISPR/Cas9 technology has revolutionized genome editing in mice, allowing for simple and rapid development of knockouts and knockins. CRISPR relies on small guide RNAs that direct the RNA‐guided nuclease Cas9 to a designated genomic site… read more here.
Keywords: genome editing; crispr cas9; cas9 technology; cas9 ... See more keywords
Efficient CRISPR/Cas9‐Mediated Mutagenesis in Primary Murine T Lymphocytes
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DOI: 10.1002/cpim.62
Abstract: The ability to alter gene expression directly in T lymphocytes has provided a powerful tool for understanding T cell biology, signaling, and function. Manipulation of T cell clones and primary T cells has been accomplished… read more here.
Keywords: mediated mutagenesis; mutagenesis primary; crispr cas9; cas9 mediated ... See more keywords
Chemical Modification and Transformation Strategies of Guide RNAs in CRISPR-Cas9 Gene Editing Systems.
Sign Up to like & getrecommendations! Published in 2021 at "ChemPlusChem"
DOI: 10.1002/cplu.202000785
Abstract: The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated protein 9) is a most powerful tool and has been widely used in gene editing and gene regulation since its discovery. However, wild-type CRISPR-Cas9 suffers from… read more here.
Keywords: guide rnas; gene editing; crispr cas9; gene ... See more keywords
Single nucleotide editing without DNA cleavage using CRISPR/Cas9‐deaminase in the sea urchin embryo
Sign Up to like & getrecommendations! Published in 2017 at "Developmental Dynamics"
DOI: 10.1002/dvdy.24586
Abstract: Background: A single base pair mutation in the genome can result in many congenital disorders in humans. The recent gene editing approach using CRISPR/Cas9 has rapidly become a powerful tool to replicate or repair such… read more here.
Keywords: crispr cas9; single nucleotide; nucleotide editing; sea urchin ... See more keywords
Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells
Sign Up to like & getrecommendations! Published in 2023 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202200047
Abstract: CRISPR/Cas9‐mediated targeted gene integration (TI) has been used to generate recombinant mammalian cell lines with predictable transgene expression. Identifying genomic hot spots that render high and stable transgene expression and knock‐in (KI) efficiency is critical… read more here.
Keywords: crispr cas9; ubiquitous chromatin; integration; cas9 ... See more keywords
CRISPR/Cas9‐mediated sheep MSTN gene knockout and promote sSMSCs differentiation
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.27474
Abstract: Myostatin (MSTN) is an important gene involved in the regulation of embryonic muscle cells and adult muscle development; it has a good application prospect in transgenic animal production by improving the yield of muscle. The… read more here.
Keywords: mstn gene; mstn; crispr cas9; gene knockout ... See more keywords
The implication of CRISPR/Cas9 genome editing technology in combating human oncoviruses
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Medical Virology"
DOI: 10.1002/jmv.25292
Abstract: It is evidenced that 20% of all tumors in humans are caused by oncoviruses, including human papilloma viruses, Epstein‐Barr virus, Kaposi sarcoma virus, human polyomaviruses, human T‐lymphotrophic virus‐1, and hepatitis B and C viruses. Human… read more here.
Keywords: virus; crispr cas9; cas9; genome editing ... See more keywords
Lipopolymeric nanocarrier enables effective delivery of CRISPR/Cas9 expressing plasmid.
Sign Up to like & getrecommendations! Published in 2023 at "Macromolecular rapid communications"
DOI: 10.1002/marc.202300101
Abstract: CRISPR/Cas9 has proven its accuracy and precision for gene editing by making a double-strand break at the predetermined nucleic acid sequence. Despite being a mainstream gene editing tool, CRISPR/Cas9 has limitations for its in vivo… read more here.
Keywords: crispr cas9; nanocarrier enables; cas9; lipopolymeric nanocarrier ... See more keywords