Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep45775
Abstract: The development of programmable nucleases has enabled the application of new genome engineering strategies for cellular immunotherapy. While targeted nucleases have mostly been used to knock-out or knock-in genes in immune cells, the scarless exchange… read more here.
Keywords: mhc specificity; reprogramming mhc; crispr cas9; cas9 assisted ... See more keywords
μLAS technology for DNA isolation coupled to Cas9-assisted targeting for sequencing and assembly of a 30 kb region in plant genome
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz632
Abstract: Abstract Cas9-assisted targeting of DNA fragments in complex genomes is viewed as an essential strategy to obtain high-quality and continuous sequence data. However, the purity of target loci selected by pulsed-field gel electrophoresis (PFGE) has… read more here.
Keywords: dna; assisted targeting; cas9 assisted; technology ... See more keywords
CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application in N-Acetylglucosamine Production
Sign Up to like & getrecommendations! Published in 2019 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01367-19
Abstract: CRISPR/Cas9-assisted recombineering is restricted in lactic acid bacteria because of the lack of available antibiotics and vectors. In this study, a seamless genome editing method was carried out in Lactobacillus plantarum using CRISPR/Cas9-assisted double-stranded DNA… read more here.
Keywords: glcnac; cas9 assisted; lactobacillus plantarum; crispr cas9 ... See more keywords
Construction and application of a CRISPR/Cas9-assisted genomic editing system for Corynebacterium glutamicum
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DOI: 10.1186/s13568-021-01231-7
Abstract: Corynebacterium glutamicum is widely used as microbial cell factory for various bioproducts, but its genomic editing efficiency needs to be improved. In this study, a highly efficient CRISPR/Cas9-assisted genomic editing system for C. glutamicum was… read more here.
Keywords: system; crispr cas9; cas9 assisted; glutamicum ... See more keywords