Generation of reproductive transgenic pigs of a CRISPR‐Cas9‐based oncogene‐inducible system by somatic cell nuclear transfer
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100434
Abstract: Alternative cancer models that are close to humans are required to create more valuable preclinical results during oncology studies. Here, a new onco‐pig model via developing a CRISPR‐Cas9‐based Conditional Polycistronic gene expression Cassette (CRI‐CPC) system… read more here.
Keywords: crispr cas9; system; cri cpc; cas9 based ... See more keywords
CRISPR/Cas9-Based Gene Dropout Screens.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8876-1_15
Abstract: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-based technology enables efficient and precise perturbations of target genomic sites. Combining the endonuclease Cas9 and a pooled guide RNA library allows for systematic screenings of genes associated with… read more here.
Keywords: cas9 based; crispr cas9; gene dropout; dropout screens ... See more keywords
CRISPR/Cas9-based genome editing of the filamentous fungi: the state of the art
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8497-9
Abstract: In recent years, a variety of genetic tools have been developed and applied to various filamentous fungi, which are widely applied in agriculture and the food industry. However, the low efficiency of gene targeting has… read more here.
Keywords: cas9 based; crispr cas9; filamentous fungi; genome editing ... See more keywords
Mobile CRISPR-Cas9 based anti-phage system in E. coli
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers of Chemical Science and Engineering"
DOI: 10.1007/s11705-022-2141-7
Abstract: Escherichia coli is one of the most important microbial cell factories, but infection by bacteriophages in the environment may have a huge impact on its application in industrial production. Here, we developed a mobile CRISPR-Cas9… read more here.
Keywords: crispr cas9; infection; phage; cas9 based ... See more keywords
CRISPR/Cas9-based precision tagging of essential genes in bloodstream form African trypanosomes.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular and biochemical parasitology"
DOI: 10.1016/j.molbiopara.2022.111476
Abstract: Proteins of interest are frequently expressed with a fusion-tag to facilitate experimental analysis. In trypanosomatids, which are typically diploid, a tag-encoding DNA fragment is typically fused to one native allele. However, since recombinant cells represent… read more here.
Keywords: bloodstream form; african trypanosomes; cas9 based; form african ... See more keywords
Design, execution, and analysis of CRISPR–Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans
Sign Up to like & getrecommendations! Published in 2019 at "Nature Protocols"
DOI: 10.1038/s41596-018-0122-6
Abstract: The study of fungal pathogens is of immediate importance, yet progress is hindered by the technical challenges of genetic manipulation. For Candida species, their inability to maintain plasmids, unusual codon usage, and inefficient homologous recombination… read more here.
Keywords: cas9 based; crispr cas9; candida; candida albicans ... See more keywords
A CRISPR-Cas9 based shuffle system for endogenous histone H3 and H4 combinatorial mutagenesis
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-82774-4
Abstract: Post-translational modifications of histone proteins greatly impact gene expression and cell fate decisions in eukaryotes. To study these, it is important to develop a convenient, multiplex, and efficient method to precisely introduce mutations to histones.… read more here.
Keywords: cas9 based; based shuffle; crispr cas9; shuffle system ... See more keywords
Genome Editing of the Anaerobic Thermophile Thermoanaerobacter ethanolicus Using Thermostable Cas9
Sign Up to like & getrecommendations! Published in 2020 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01773-20
Abstract: Interest in thermophilic microorganisms as emerging metabolic engineering platforms to produce biofuels and chemicals has surged. Thermophilic microbes for biofuels have attracted great attention, due to their tolerance of high temperature and wide range of… read more here.
Keywords: cas9 based; genome editing; thermostable cas9; gene ... See more keywords
CRISPR/Cas9-Based RGEN-ISL Allows the Simultaneous and Specific Visualization of Proteins, DNA Repeats, and Sites of DNA Replication
Sign Up to like & getrecommendations! Published in 2019 at "Cytogenetic and Genome Research"
DOI: 10.1159/000502600
Abstract: Visualizing the spatiotemporal organization of the genome will improve our understanding of how chromatin structure and function are intertwined. Here, we describe a further development of the CRISPR/Cas9-based RNA-guided endonuclease-in situ labeling (RGEN-ISL) method. RGEN-ISL… read more here.
Keywords: cas9 based; microscopy; crispr cas9; rgen isl ... See more keywords
Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis
Sign Up to like & getrecommendations! Published in 2022 at "PLOS ONE"
DOI: 10.1371/journal.pone.0278972
Abstract: Chlamydomonas reinhardtii is an important model organism for the study of many cellular processes, and protein tagging is an increasingly indispensable tool for these studies. To circumvent the disadvantages of conventional approaches in creating a… read more here.
Keywords: crispr cas9; protein tagging; situ; chlamydomonas reinhardtii ... See more keywords
Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2020.00882
Abstract: The speed of reprogramming technologies evolution is rising dramatically in modern science. Both the scientific community and health workers depend on such developments due to the lack of safe autogenic cells and tissues for regenerative… read more here.
Keywords: cas9 based; cell reprogramming; crispr; reprogramming crispr ... See more keywords