RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a
Sign Up to like & getrecommendations! Published in 2017 at "Cell reports"
DOI: 10.1016/j.celrep.2017.11.100
Abstract: SUMMARY CRISPR-Cas systems provide bacteria and archaea with sequence-specific protection against invading mobile genetic elements. In the presence of divalent metal ions, Cas9 and Cas12a (formerly Cpf1) proteins target and cleave DNA that is complementary… read more here.
Keywords: independent dna; rna independent; cas9 cas12a; dna cleavage ... See more keywords
Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical Society Transactions"
DOI: 10.1042/bst20190563
Abstract: Discovered as an adaptive immune system of prokaryotes, CRISPR–Cas provides many promising applications. DNA-cleaving Cas enzymes like Cas9 and Cas12a, are of great interest for genome editing. The specificity of these DNA nucleases is determined… read more here.
Keywords: crispr; cas9 cas12a; dna cleavage; dna ... See more keywords