Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On‐ and Off‐Target Damage
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DOI: 10.1002/bies.202000047
Abstract: Large deletions and genomic re‐arrangements are increasingly recognized as common products of double‐strand break repair at Clustered Regularly Interspaced, Short Palindromic Repeats ‐ CRISPR associated protein 9 (CRISPR/Cas9) on‐target sites. Together with well‐known off‐target editing… read more here.
Keywords: crispr cas9; cas9 target; cas9 editing; target ... See more keywords
Plasmid-based complementation of large deletions in Phaeodactylum tricornutum biosynthetic genes generated by Cas9 editing
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DOI: 10.1038/s41598-020-70769-6
Abstract: The model diatom Phaeodactylum tricornutum is an attractive candidate for synthetic biology applications. Development of auxotrophic strains of P. tricornutum would provide alternative selective markers to commonly used antibiotic resistance genes. Here, using CRISPR/Cas9, we… read more here.
Keywords: cas9 editing; complementation; plasmid based; phaeodactylum tricornutum ... See more keywords
Easy quantification of template-directed CRISPR/Cas9 editing
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DOI: 10.1093/nar/gky164
Abstract: Abstract Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must… read more here.
Keywords: crispr cas9; template; cas9 editing; directed crispr ... See more keywords
Generation of genetically tailored porcine liver cancer cells by CRISPR/Cas9 editing.
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DOI: 10.2144/btn-2020-0119
Abstract: Pigs provide a valuable large animal model for several diseases due to their similarity with humans in anatomy, physiology, genetics and drug metabolism. We recently generated a porcine model for TP53R167H and KRASG12D driven hepatocellular… read more here.
Keywords: crispr cas9; cells crispr; tailored porcine; cas9 editing ... See more keywords
CRISPR/Cas9 Editing for Gaucher Disease Modelling
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DOI: 10.3390/ijms21093268
Abstract: Gaucher disease (GD) is an autosomal recessive lysosomal storage disorder caused by mutations in the acid β-glucosidase gene (GBA1). Besides causing GD, GBA1 mutations constitute the main genetic risk factor for developing Parkinson’s disease. The… read more here.
Keywords: gaucher disease; crispr cas9; cas9 editing; disease ... See more keywords