Solid-State Nanopore Real-Time Assay for Monitoring Cas9 Endonuclease Reactivity
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DOI: 10.1101/2024.09.20.612695
Abstract: The field of nanopore sensing is now moving beyond nucleic acid sequencing. An exciting new avenue is the use of nanopore platforms for the monitoring of biochemical reactions. Biological nanopores have been used to investigate… read more here.
Keywords: solid state; real time; cas9 endonuclease; state nanopore ... See more keywords
Cas9 endonuclease and off-target activity
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DOI: 10.1126/science.357.6350.467-q
Abstract: Genome Sequencing The RNA-guided endonuclease Cas9 has brought about a revolution in genetic engineering and genome editing. However, issues with the system include the off-target activity of the endonuclease and promiscuous cleavage, making accuracy in… read more here.
Keywords: cas9 endonuclease; endonuclease; target activity; endonuclease target ... See more keywords