Gene editing provides a tool to investigate genes involved in reproduction of pigs.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular reproduction and development"
DOI: 10.1002/mrd.23620
Abstract: CRISPR-Cas9 gene editing technology provides a method to generate loss-of-function studies to investigate, in vivo, the specific role of specific genes in regulation of reproduction. With proper design and selection of guide RNAs (gRNA) designed… read more here.
Keywords: crispr cas9; gene editing; cas9 gene; editing technology ... See more keywords
CRISPR/Cas9 Gene editing of RyR2 in human stem cell-derived cardiomyocytes provides a novel approach in investigating dysfunctional Ca2+ signaling.
Sign Up to like & getrecommendations! Published in 2018 at "Cell calcium"
DOI: 10.1016/j.ceca.2018.04.009
Abstract: Type-2 ryanodine receptors (RyR2s) play a pivotal role in cardiac excitation-contraction coupling by releasing Ca2+ from sarcoplasmic reticulum (SR) via a Ca2+ -induced Ca2+ release (CICR) mechanism. Two strategies have been used to study the… read more here.
Keywords: gene editing; crispr cas9; cas9 gene; ca2 ... See more keywords
Utilization of CRISPR/Cas9 gene editing in cellular therapies for lymphoid malignancies.
Sign Up to like & getrecommendations! Published in 2020 at "Immunology letters"
DOI: 10.1016/j.imlet.2020.07.003
Abstract: The ability to change the genetic information of immune cells is a powerful tool for basic and clinical settings. CRISPR/Cas9 gene editing technology by providing an efficient approach has accelerated immune cell therapy of cancers.… read more here.
Keywords: crispr cas9; gene editing; cas9 gene; lymphoid malignancies ... See more keywords
Reversible RNA acylation for control of CRISPR–Cas9 gene editing†
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.1039/c9sc03639c
Abstract: We report the development of post-transcriptional chemical methods that enable control over CRISPR–Cas9 gene editing activity both in in vitro assays and in living cells. We show that an azide-substituted acyl imidazole reagent (NAI-N3) efficiently… read more here.
Keywords: gene editing; crispr cas9; cas9 gene; control crispr ... See more keywords
Generation of ‘designer erythroblasts’ lacking one or more blood group systems from CRISPR/Cas9 gene‐edited human‐induced pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Cellular and Molecular Medicine"
DOI: 10.1111/jcmm.16872
Abstract: Despite the recent advancements in transfusion medicine, red blood cell (RBC) alloimmunization remains a challenge for multiparous women and chronically transfused patients. At times, diagnostic laboratories depend on difficult‐to‐procure rare reagent RBCs for the identification… read more here.
Keywords: blood group; crispr cas9; medicine; cas9 gene ... See more keywords
ORFV can carry TRAP gene expression via intracellular CRISPR/Cas9 gene editing technology.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of virological methods"
DOI: 10.2139/ssrn.4120630
Abstract: Orf is an acute and highly contracted human and animal infection caused by orf virus (ORFV), which mainly affects sheep, goats, and other species. Clinically, opportunistic or conditional pathogens such as Staphylococcus aureus (S. aureus)… read more here.
Keywords: crispr cas9; gene editing; trap gene; cas9 gene ... See more keywords
The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2018.00285
Abstract: Campylobacter jejuni is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR–cas9 system, which is used as a gene editing technique in different organisms. However, its role in… read more here.
Keywords: campylobacter jejuni; virulence; cas9; cas9 gene ... See more keywords
Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22136724
Abstract: The spotted-wing Drosophila (Drosophila suzukii Matsumura) is native to eastern Asia, but has become a global threat to fruit production. In recent years, CRISPR/Cas9 targeting was established in this species allowing for functional genomic and… read more here.
Keywords: crispr cas9; cas9 gene; efficient temperature; gene ... See more keywords
Efficient Cross-Screening and Characterization of Monoclonal Antibodies against Marek’s Disease Specific Meq Oncoprotein Using CRISPR/Cas9-Gene-Edited Viruses
Sign Up to like & getrecommendations! Published in 2023 at "Viruses"
DOI: 10.3390/v15040817
Abstract: Marek’s disease (MD) caused by pathogenic Marek’s disease virus type 1 (MDV−1) is one of the most important neoplastic diseases of poultry. MDV−1-encoded unique Meq protein is the major oncoprotein and the availability of Meq-specific… read more here.
Keywords: meq; crispr cas9; mdv; marek disease ... See more keywords