Gene editing provides a tool to investigate genes involved in reproduction of pigs.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular reproduction and development"
DOI: 10.1002/mrd.23620
Abstract: CRISPR-Cas9 gene editing technology provides a method to generate loss-of-function studies to investigate, in vivo, the specific role of specific genes in regulation of reproduction. With proper design and selection of guide RNAs (gRNA) designed… read more here.
Keywords: crispr cas9; gene editing; cas9 gene; editing technology ... See more keywords
CRISPR/Cas9 Gene editing of RyR2 in human stem cell-derived cardiomyocytes provides a novel approach in investigating dysfunctional Ca2+ signaling.
Sign Up to like & getrecommendations! Published in 2018 at "Cell calcium"
DOI: 10.1016/j.ceca.2018.04.009
Abstract: Type-2 ryanodine receptors (RyR2s) play a pivotal role in cardiac excitation-contraction coupling by releasing Ca2+ from sarcoplasmic reticulum (SR) via a Ca2+ -induced Ca2+ release (CICR) mechanism. Two strategies have been used to study the… read more here.
Keywords: gene editing; crispr cas9; cas9 gene; ca2 ... See more keywords
Utilization of CRISPR/Cas9 gene editing in cellular therapies for lymphoid malignancies.
Sign Up to like & getrecommendations! Published in 2020 at "Immunology letters"
DOI: 10.1016/j.imlet.2020.07.003
Abstract: The ability to change the genetic information of immune cells is a powerful tool for basic and clinical settings. CRISPR/Cas9 gene editing technology by providing an efficient approach has accelerated immune cell therapy of cancers.… read more here.
Keywords: crispr cas9; gene editing; cas9 gene; lymphoid malignancies ... See more keywords
Near-Infrared Optogenetic Nanosystem for Spatiotemporal Control of CRISPR-Cas9 Gene Editing and Synergistic Photodynamic Therapy.
Sign Up to like & getrecommendations! Published in 2024 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.4c18656
Abstract: Controlling CRISPR/Cas9 gene editing at the spatiotemporal resolution level, especially for in vivo applications, remains a great challenge. Here, we developed a near-infrared (NIR) light-activated nanophotonic system (UCPP) for controlled CRISPR-Cas9 gene editing and synergistic… read more here.
Keywords: gene editing; cas9 gene; gene; crispr cas9 ... See more keywords
Reversible RNA acylation for control of CRISPR–Cas9 gene editing†
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.1039/c9sc03639c
Abstract: We report the development of post-transcriptional chemical methods that enable control over CRISPR–Cas9 gene editing activity both in in vitro assays and in living cells. We show that an azide-substituted acyl imidazole reagent (NAI-N3) efficiently… read more here.
Keywords: gene editing; crispr cas9; cas9 gene; control crispr ... See more keywords
Generation of ‘designer erythroblasts’ lacking one or more blood group systems from CRISPR/Cas9 gene‐edited human‐induced pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Cellular and Molecular Medicine"
DOI: 10.1111/jcmm.16872
Abstract: Despite the recent advancements in transfusion medicine, red blood cell (RBC) alloimmunization remains a challenge for multiparous women and chronically transfused patients. At times, diagnostic laboratories depend on difficult‐to‐procure rare reagent RBCs for the identification… read more here.
Keywords: blood group; crispr cas9; medicine; cas9 gene ... See more keywords
Abstract 4141227: Characterization of NIMA-related kinase 7 (NEK7)-NLRP3 inflammasome complex in macrophages by combining CRISPR/Cas9 gene-editing with comprehensive proteomic
Sign Up to like & getrecommendations! Published in 2024 at "Circulation"
DOI: 10.1161/circ.150.suppl_1.4141227
Abstract: Background: The aberrant activation of the NLRP3 inflammasome in macrophages promotes chronic inflammation in cardiovascular disease (CVD). NEK7 is essential to the NLRP3 inflammasome’s function, however, its role in inflammasome-mediated macrophage activation has yet to… read more here.
Keywords: inflammasome; nek7; nlrp3 inflammasome; cas9 gene ... See more keywords
Integrating malaria vaccine and CRISPR/Cas9 gene drive: a comprehensive strategy for accelerated malaria eradication
Sign Up to like & getrecommendations! Published in 2025 at "Malaria Journal"
DOI: 10.1186/s12936-025-05243-7
Abstract: Malaria remains a significant public health challenge, particularly in low- and middle-income countries, despite ongoing efforts to eradicate the disease. Recent advancements, including the rollout of malaria vaccines, such as RTS,S/AS01 and R21/Matrix-M™, offer new… read more here.
Keywords: gene; cas9 gene; gene drive; crispr cas9 ... See more keywords
ORFV can carry TRAP gene expression via intracellular CRISPR/Cas9 gene editing technology.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of virological methods"
DOI: 10.2139/ssrn.4120630
Abstract: Orf is an acute and highly contracted human and animal infection caused by orf virus (ORFV), which mainly affects sheep, goats, and other species. Clinically, opportunistic or conditional pathogens such as Staphylococcus aureus (S. aureus)… read more here.
Keywords: crispr cas9; gene editing; trap gene; cas9 gene ... See more keywords
The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2018.00285
Abstract: Campylobacter jejuni is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR–cas9 system, which is used as a gene editing technique in different organisms. However, its role in… read more here.
Keywords: campylobacter jejuni; virulence; cas9; cas9 gene ... See more keywords
Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli
Sign Up to like & getrecommendations! Published in 2024 at "Genes"
DOI: 10.3390/genes15060668
Abstract: Broccoli, a popular international Brassica oleracea crop, is an important export vegetable in China. Broccoli is not only rich in protein, vitamins, and minerals but also has anticancer and antiviral activities. Recently, an Agrobacterium-mediated transformation… read more here.
Keywords: broccoli; gene editing; cas9 gene; gene ... See more keywords