Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
Sign Up to like & getrecommendations! Published in 2019 at "Advanced materials"
DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
CRISPR–Cas9 in genome editing: Its function and medical applications
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.27476
Abstract: The targeted genome modification using RNA‐guided nucleases is associated with several advantages such as a rapid, easy, and efficient method that not only provides the manipulation and alteration of genes and functional studies for researchers,… read more here.
Keywords: genome editing; cas9 genome; crispr cas9; editing function ... See more keywords
CRISPR/Cas9 Genome Editing in Embryonic Stem Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-4035-6_15
Abstract: Targeted mutagenesis is required to evaluate the function of DNA segments across the genome. In recent years the CRISPR/Cas9 technology has been widely used for functional genome studies and is partially replacing classical homologous recombination… read more here.
Keywords: genome editing; cas9 genome; crispr cas9; embryonic stem ... See more keywords
CRISPR/Cas9 genome editing in ergot fungus Claviceps purpurea.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2020.09.028
Abstract: Claviceps purpurea is a filamentous fungus well known as a widespread plant pathogen, but it is also an important ergot alkaloid producer exploited by the pharmaceutic industry. In this work, we demonstrated that CRISPR/Cas9 can… read more here.
Keywords: genome editing; editing ergot; cas9 genome; crispr cas9 ... See more keywords
Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-26097-y
Abstract: Karyotype alterations have emerged as on-target complications from CRISPR-Cas9 genome editing. However, the events that lead to these karyotypic changes in embryos after Cas9-treatment remain unknown. Here, using imaging and single-cell genome sequencing of 8-cell… read more here.
Keywords: genome editing; cas9 genome; crispr cas9; genome ... See more keywords
Advances in CRISPR/Cas9 Genome Editing for the Treatment of Muscular Dystrophies.
Sign Up to like & getrecommendations! Published in 2023 at "Human gene therapy"
DOI: 10.1089/hum.2023.059
Abstract: Muscular dystrophies (MDs) comprise a diverse group of inherited disorders characterized by progressive muscle loss and weakness. Given the genetic etiology underlying MDs, researchers have explored the potential of CRISPR/Cas9 genome editing as a therapeutic… read more here.
Keywords: genome editing; crispr cas9; cas9 genome; muscular dystrophies ... See more keywords
Using CRISPR/Cas9 genome editing in human iPSCs for deciphering the pathogenicity of a novel CCM1 transcription start site deletion
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.953048
Abstract: Cerebral cavernous malformations are clusters of aberrant vessels that can lead to severe neurological complications. Pathogenic loss-of-function variants in the CCM1, CCM2, or CCM3 gene are associated with the autosomal dominant form of the disease.… read more here.
Keywords: crispr cas9; deletion; cas9 genome; using crispr ... See more keywords
CRISPR/Cas9 Genome-Editing Technology and Potential Clinical Application in Gastric Cancer
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13112029
Abstract: Gastric cancer is the subject of clinical and basic studies due to its high incidence and mortality rates worldwide. Due to the diagnosis occurring in advanced stages and the classic treatment methodologies such as gastrectomy… read more here.
Keywords: crispr cas9; cas9 genome; editing technology; gastric cancer ... See more keywords
Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in Flammulina filiformis
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Fungi"
DOI: 10.3390/jof8070693
Abstract: Flammulina filiformis, previously known as Asian Flammulina velutipes, is one of the most commercially important edible fungi, with nutritional value and medicinal properties worldwide. However, precision genome editing using CRISPR/Cas9, which is a revolutionary technology… read more here.
Keywords: genome editing; crispr cas9; dual sgrnas; cas9 genome ... See more keywords