Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell Reports"
DOI: 10.1007/s00299-019-02488-w
Abstract: Key message A novel and robust lipofection-mediated transfection approach for the use of DNA-free Cas9/gRNA RNP for gene editing has demonstrated efficacy in plant cells. Abstract Precise genome editing has been revolutionized by CRISPR/Cas9 systems.… read more here.
Keywords: lipofection; plant; delivery; cas9 grna ... See more keywords
Cas9/gRNA-Mediated Mutations in PtrFLA40 and PtrFLA45 Reveal Redundant Roles in Modulating Wood Cell Size and SCW Synthesis in Poplar
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24010427
Abstract: Fasciclin-like arabinogalactan proteins (FLAs) play an important role in plant development and adaptation to the environment. However, the roles of FLAs in wood formation remain poorly understood. Here, we identified a total of 50 PtrFLA… read more here.
Keywords: ptrfla40 ptrfla45; wood; cell size; cas9 grna ... See more keywords