Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks
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DOI: 10.1016/j.molcel.2018.04.016
Abstract: Summary The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little is known about the kinetics and fidelity of the double-strand break (DSB) repair process that follows a Cas9 cutting event in… read more here.
Keywords: kinetics fidelity; double strand; cas9 induced; cas9 ... See more keywords
Functional analysis of the ABCs of eye color in Helicoverpa armigera with CRISPR/Cas9-induced mutations
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DOI: 10.1038/srep40025
Abstract: Many insect pigments are localized in subcellular pigment granules, and transport of pigment precursors from the cytoplasm is accomplished by ABC proteins. Drosophila melanogaster has three half-transporter genes (white, scarlet, and brown, all affecting eye… read more here.
Keywords: induced mutations; helicoverpa armigera; crispr cas9; cas9 induced ... See more keywords
CRISPRthripsis: The Risk of CRISPR/Cas9-induced Chromothripsis in Gene Therapy
Sign Up to like & getrecommendations! Published in 2022 at "Stem Cells Translational Medicine"
DOI: 10.1093/stcltm/szac064
Abstract: Abstract The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 nuclease system has allowed the generation of disease models and the development of therapeutic approaches for many genetic and non-genetic disorders. However, the generation of large… read more here.
Keywords: crispr cas9; induced chromothripsis; cas9 induced; crispr ... See more keywords
Marker Recycling in Candida albicans through CRISPR-Cas9-Induced Marker Excision
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DOI: 10.1128/msphere.00050-17
Abstract: It is critical to be able to alter genes in order to elucidate their functions. These alterations often rely upon markers that allow selection for a rare cell in a population that has incorporated a… read more here.
Keywords: crispr cas9; selection; marker; cas9 induced ... See more keywords
Generation and Characterization of a CRISPR/Cas9—Induced 3-mst Deficient Zebrafish
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DOI: 10.3390/biom10020317
Abstract: 3-mercaptopyruvate sulfurtransferase (3-MST) is an enzyme capable of synthesizing hydrogen sulfide (H2S) and polysulfides. In spite of its ubiquitous presence in mammalian cells, very few studies have investigated its contribution to homeostasis and disease development,… read more here.
Keywords: induced mst; cas9 induced; deficient zebrafish; mst deficient ... See more keywords
CRISPR/Cas9-Induced Knockout of Sting Increases Susceptibility of Zebrafish to Bacterial Infection
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DOI: 10.3390/biom13020324
Abstract: Stimulator of interferon genes (STING) is an adapter protein that is activated when cyclic dinucleotides (CDNs) are present. CDNs originate from the cytosolic DNA of both pathogens and hosts. STING activation promotes efficient immune responses… read more here.
Keywords: crispr cas9; infection; pathway related; sting pathway ... See more keywords