Efficient CRISPR/Cas9‐Mediated Mutagenesis in Primary Murine T Lymphocytes
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Immunology"
DOI: 10.1002/cpim.62
Abstract: The ability to alter gene expression directly in T lymphocytes has provided a powerful tool for understanding T cell biology, signaling, and function. Manipulation of T cell clones and primary T cells has been accomplished… read more here.
Keywords: mediated mutagenesis; mutagenesis primary; crispr cas9; cas9 mediated ... See more keywords
Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells
Sign Up to like & getrecommendations! Published in 2023 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202200047
Abstract: CRISPR/Cas9‐mediated targeted gene integration (TI) has been used to generate recombinant mammalian cell lines with predictable transgene expression. Identifying genomic hot spots that render high and stable transgene expression and knock‐in (KI) efficiency is critical… read more here.
Keywords: crispr cas9; ubiquitous chromatin; integration; cas9 ... See more keywords
CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9024-5_9
Abstract: State-of-the-art strain engineering techniques for the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) include overexpression of endogenous and heterologous genes and deletion of host genes. For efficient gene deletion, methods such as the split-marker technique have… read more here.
Keywords: crispr cas9; cas9 mediated; biology; pichia pastoris ... See more keywords
CRISPR/Cas9-Mediated Targeted Mutagenesis in Wheat Doubled Haploids.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9865-4_15
Abstract: CRISPR/Cas9-based genome editing technology has the potential to revolutionize agriculture, but many plant species and/or genotypes are recalcitrant to conventional transformation methods. Additionally, the long generation time of crop plants poses a significant obstacle to… read more here.
Keywords: crispr; wheat; crispr cas9; mediated targeted ... See more keywords
Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2018 at "World Journal of Microbiology and Biotechnology"
DOI: 10.1007/s11274-018-2537-1
Abstract: Genome editing using engineered nucleases has rapidly transformed from a niche technology to a mainstream method used in various host cells. Its widespread adoption has been largely developed by the emergence of the clustered regularly… read more here.
Keywords: mediated genome; crispr cas9; cas9 mediated; genome editing ... See more keywords
CRISPR/Cas9-mediated knockout of two eye pigmentation genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).
Sign Up to like & getrecommendations! Published in 2018 at "Insect biochemistry and molecular biology"
DOI: 10.1016/j.ibmb.2017.12.003
Abstract: The brown planthopper Nilaparvata lugens is one of the most destructive insect pests in Asia, demonstrating high fertility and causing huge crop losses by sucking sap of rice as well as transmitting viruses. However, functional… read more here.
Keywords: crispr cas9; nilaparvata lugens; planthopper nilaparvata; brown planthopper ... See more keywords
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus.
Sign Up to like & getrecommendations! Published in 2019 at "Stem cell research"
DOI: 10.1016/j.scr.2018.101378
Abstract: STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro… read more here.
Keywords: line; homozygous crispr; generation homozygous; crispr cas9 ... See more keywords
Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing.
Sign Up to like & getrecommendations! Published in 2018 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00760
Abstract: CRISPR/Cas9-mediated gene editing may involve nonhomologous end-joining to create various insertion/deletions (indels) or may employ homologous recombination to modify precisely the target DNA sequence. Our understanding of these processes has been guided by earlier studies… read more here.
Keywords: gene editing; mediated gene; site specific; gene ... See more keywords
Crispr/Cas9 Mediated Inactivation of Argonaute 2 Reveals its Differential Involvement in Antiviral Responses
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-01050-6
Abstract: RNA silencing constitutes an important antiviral mechanism in plants. Small RNA guided Argonaute proteins fulfill essential role in this process by acting as executors of viral restriction. Plants encode multiple Argonaute proteins of which several… read more here.
Keywords: crispr cas9; antiviral responses; involvement antiviral; cas9 mediated ... See more keywords
CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-08596-5
Abstract: Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis… read more here.
Keywords: mediated one; crispr cas9; disabling pancreatogenesis; cas9 mediated ... See more keywords
Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis
Sign Up to like & getrecommendations! Published in 2022 at "Open Biology"
DOI: 10.1098/rsob.210361
Abstract: CRISPR/Cas9-mediated genome editing has become an extremely powerful technique used to modify gene expression in many organisms, including parasitic protists. Giardia intestinalis, a protist parasite that infects approximately 280 million people around the world each… read more here.
Keywords: crispr cas9; intestinalis; cas9; cas9 mediated ... See more keywords