CRISPR-Cas9 Mutagenesis in Xenopus tropicalis for Phenotypic Analyses in the F0 Generation and Beyond.
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DOI: 10.1101/pdb.prot106971
Abstract: CRISPR-Cas9 mutagenesis is being widely used to create targeted loss-of-function mutations in the diploid frog Xenopus tropicalis Here we describe a simple mutagenesis protocol using microinjection of Cas9 protein or mRNA, together with synthetic guide… read more here.
Keywords: cas9 mutagenesis; crispr cas9; xenopus tropicalis; mutagenesis ... See more keywords
Chromatin accessibility is associated with CRISPR-Cas9 efficiency in the zebrafish (Danio rerio)
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DOI: 10.1371/journal.pone.0196238
Abstract: CRISPR-Cas9 technology is routinely applied for targeted mutagenesis in model organisms and cell lines. Recent studies indicate that the prokaryotic CRISPR-Cas9 system is affected by eukaryotic chromatin structures. Here, we show that the likelihood of… read more here.
Keywords: cas9 mutagenesis; crispr cas9; chromatin; mutagenesis ... See more keywords