Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs
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DOI: 10.1038/s41598-021-89312-2
Abstract: Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines. However, in typical protocols, mutations are intentionally introduced into the donor template to avoid the cleavage of donor template or re-cleavage of the successfully… read more here.
Keywords: guided cas9; genome editing; single guided; human ipscs ... See more keywords
SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds
Sign Up to like & getrecommendations! Published in 2020 at "Genome Biology"
DOI: 10.1186/s13059-020-02051-x
Abstract: We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to… read more here.
Keywords: genome editing; crispr; editing plants; rna ... See more keywords