A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes.
Sign Up to like & getrecommendations! Published in 2021 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2021.03.017
Abstract: Media and feed optimization have fueled many-fold improvements in mammalian biopharmaceutical production, but genome editing offers an emerging avenue for further enhancing cell metabolism and bioproduction. However, the complexity of metabolism, involving thousands of genes,… read more here.
Keywords: screen chinese; crispr; crispr cas9; metabolic crispr ... See more keywords
A CRISPR/Cas9 screen in embryonic stem cells reveals that Mdm2 regulates totipotency exit
Sign Up to like & getrecommendations! Published in 2024 at "Communications Biology"
DOI: 10.1038/s42003-024-06507-9
Abstract: During early embryonic development, the transition from totipotency to pluripotency is a fundamental and critical process for proper development. However, the regulatory mechanisms governing this transition remain elusive. Here, we conducted a comprehensive genome-wide CRISPR/Cas9… read more here.
Keywords: totipotency; cas9 screen; crispr cas9; stem cells ... See more keywords
An in vivo barcoded CRISPR-Cas9 screen identifies Ncoa4-mediated ferritinophagy as a dependence in Tet2-deficient hematopoiesis.
Sign Up to like & getrecommendations! Published in 2025 at "Blood"
DOI: 10.1182/blood.2024028033
Abstract: TET2 is among the most commonly mutated genes in both clonal hematopoiesis and myeloid malignancies, thus, the ability to identify selective dependencies in TET2 deficient cells has broad translational significance. Here, we identify regulators of… read more here.
Keywords: cas9 screen; hematopoiesis; tet2 deficient; tet2 ... See more keywords