Genome-wide CRISPR-Cas9 screening in mammalian cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.04.015
Abstract: Forward genetic screens are a powerful and unbiased approach for uncovering the genetic basis behind a specific phenotype. Genome-wide mutagenesis followed by phenotypic screening represents the ultimate manifestation of this method, directly linking biological phenomena… read more here.
Keywords: genome wide; cas9 screening; crispr; crispr cas9 ... See more keywords
Identifying novel therapeutic targets in gastric cancer using genome-wide CRISPR-Cas9 screening
Sign Up to like & getrecommendations! Published in 2022 at "Oncogene"
DOI: 10.1038/s41388-022-02177-1
Abstract: Genome-scale CRISPR-Cas9 screening technology is a powerful tool to systematically identify genes essential for cancer cell survival. Herein, TKOv3, a genome-scale CRISPR-Cas9 knock-out library, was screened in the gastric cancer (GC) cells, and relevant validation… read more here.
Keywords: cancer; essential genes; cas9 screening; crispr cas9 ... See more keywords
Genome-wide CRISPR-Cas9 Screening Reveals Ubiquitous T cell Cancer Targeting via the monomorphic MHC class I related protein MR1
Sign Up to like & getrecommendations! Published in 2020 at "Nature immunology"
DOI: 10.1038/s41590-019-0578-8
Abstract: Human leukocyte antigen (HLA)-independent, T cell–mediated targeting of cancer cells would allow immune destruction of malignancies in all individuals. Here, we use genome-wide CRISPR–Cas9 screening to establish that a T cell receptor (TCR) recognized and… read more here.
Keywords: genome wide; cas9 screening; crispr cas9; wide crispr ... See more keywords
Requirement of glycosylation machinery in TLR responses revealed by CRISPR/Cas9 screening
Sign Up to like & getrecommendations! Published in 2017 at "International Immunology"
DOI: 10.1093/intimm/dxx044
Abstract: The Toll family of receptors sense microbial products and activate a defense response. The molecular machinery required for the TLR response is not yet fully understood. In the present study, we used a clustered, regularly… read more here.
Keywords: cas9 screening; response; machinery; crispr cas9 ... See more keywords