Scaffold-mediated non-viral delivery platform for CRISPR/Cas9-based genome editing.
Sign Up to like & getrecommendations! Published in 2019 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2019.04.020
Abstract: Genome editing, especially via the simple and versatile type II CRISPR/Cas9 system, offers an effective avenue to precisely control cell fate, an important aspect of tissue regeneration. Unfortunately, most CRISPR/Cas9 non-viral delivery strategies only utilise… read more here.
Keywords: genome editing; non viral; crispr cas9; cas9 sgrna ... See more keywords
A Universal GSH-Responsive Nanoplatform for the Delivery of DNA, mRNA, and Cas9/sgRNA Ribonucleoprotein.
Sign Up to like & getrecommendations! Published in 2018 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b03496
Abstract: The long-sought promise of gene therapy for the treatment of human diseases remains unfulfilled, largely hindered by the lack of an efficient and safe delivery vehicle. In this study, we have developed a universal glutathione-responsive… read more here.
Keywords: dna mrna; dna; mrna cas9; cas9 sgrna ... See more keywords
Peptide and Aptamer Decorated Delivery System for Targeting Delivery of Cas9/sgRNA Plasmid To Mediate Antitumor Genome Editing.
Sign Up to like & getrecommendations! Published in 2019 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.9b05772
Abstract: A multiple-functionalized targeting delivery system was prepared by self-assembly for efficient delivery of Cas9/sgRNA plasmids to targeted tumor cell nuclei. The Cas9/sgRNA plasmids were compacted by protamine in the presence of calcium ions to form… read more here.
Keywords: genome editing; system; targeting delivery; cas9 sgrna ... See more keywords
Points of View on the Tools for Genome/Gene Editing
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22189872
Abstract: Theoretically, a DNA sequence-specific recognition protein that can distinguish a DNA sequence equal to or more than 16 bp could be unique to mammalian genomes. Long-sequence-specific nucleases, such as naturally occurring Homing Endonucleases and artificially… read more here.
Keywords: cas9 sgrna; breakage; target; complementary strand ... See more keywords