An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system
Sign Up to like & getrecommendations! Published in 2020 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202000032
Abstract: Although the efficiency and versatility of CRISPR‐Cas9 system has been greatly improved over conventional genome editing methods such as zinc finger or TALEN, it is still time‐consuming and labor‐intensive for screening knockout/knock‐in cell clones due… read more here.
Keywords: cell lines; crispr cas9; knockout cell; cas9 system ... See more keywords
CRISPR Editing in Biological and Biomedical Investigation
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.26111
Abstract: The revolutionary technology for genome editing known as the clustered regularly interspaced short palindromic repeat (CRISPR)‐CRISPR‐associated protein 9 (Cas9) system has sparked advancements in biological and biomedical research. The scientific breakthrough of the development of… read more here.
Keywords: crispr; crispr cas9; crispr editing; cas9 system ... See more keywords
CRISPR/Cas9 system-mediated impairment of synaptobrevin/VAMP function in postmitotic hippocampal neurons
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2016.12.015
Abstract: BACKGROUND The use of the CRISPR/Cas9 system is becoming widespread, however current studies have predominantly focused on dividing cells. It is currently unknown if CRISPR/Cas9 can be used in a postmitotic setting to examine non-cell… read more here.
Keywords: crispr cas9; non cell; cas9 system; cell autonomous ... See more keywords
Harnessing nanoparticles for the efficient delivery of the CRISPR/Cas9 system
Sign Up to like & getrecommendations! Published in 2020 at "Nano Today"
DOI: 10.1016/j.nantod.2020.100895
Abstract: Abstract Exploiting bacterial DNA-acting enzymes and expanding their repertoire for genome engineering have been major technological and conceptual advances in molecular biology over the last decade. The CRISPR-Cas9 system offers many attractive superiorities, such as… read more here.
Keywords: crispr cas9; delivery crispr; cas9; cas9 system ... See more keywords
Generation of B2M bi-allelic knockout human induced pluripotent stem cells (MUSIi001-A-1) using a CRISPR/Cas9 system.
Sign Up to like & getrecommendations! Published in 2021 at "Stem cell research"
DOI: 10.1016/j.scr.2021.102551
Abstract: Allogeneic cell-based therapy is emerging as a promising approach in regenerative medicine. However, rejection of allograft due to mismatch of human leukocyte antigens (HLAs) remains a major concern after transplantation. Here, we generated a homozygous… read more here.
Keywords: crispr cas9; pluripotent stem; cas9 system; induced pluripotent ... See more keywords
Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system
Sign Up to like & getrecommendations! Published in 2022 at "Veterinary and Animal Science"
DOI: 10.1016/j.vas.2022.100241
Abstract: Highlights • Mosaicism is a serious problem for genome editing during embryogenesis.• We hypothesized that genome-editing before in vitro fertilization can increase its efficiency.• We introduced CRISPR/Cas9 system into oocytes during in vitro maturation using… read more here.
Keywords: crispr cas9; gene; vitro maturation; cas9 system ... See more keywords
Application of CRISPR/Cas9 System to Reverse ABC-Mediated Multidrug Resistance.
Sign Up to like & getrecommendations! Published in 2021 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.0c00627
Abstract: Multidrug resistance (MDR) is the main obstacle in cancer chemotherapy. ATP-binding cassette (ABC) transporters can transport a wide range of antitumor drugs out of cells, which is the most common reason in the development of… read more here.
Keywords: abc mediated; crispr cas9; resistance; cas9 system ... See more keywords
A CRISPR-Cas9 System-Mediated Genetic Disruption and Multi-fragment Assembly in Starmerella bombicola.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00582
Abstract: Gene editing technology plays an extremely significant role in synthetic biology and metabolic engineering. Traditional genetic manipulation methods, such as homologous recombination, however, are inefficient, time-consuming, and barely feasible when disrupting multiple genes simultaneously. Starmerella… read more here.
Keywords: cas9; crispr cas9; biology; cas9 system ... See more keywords
Repurposing the Endogenous CRISPR-Cas9 System for High-Efficiency Genome Editing in Lacticaseibacillus paracasei.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00374
Abstract: Lactobacilli such as Lacticaseibacillus (Lcb) paracasei are generally regarded as safe and health-promoting microbes, and have been widely applied in food and pharmaceutical industries. However, the genetic bases of their beneficial properties were mostly uncertain… read more here.
Keywords: cas9 system; crispr cas9; efficiency; crispr ... See more keywords
Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00411
Abstract: The mitochondria DNA (mtDNA) editing tool, zinc finger nucleases (ZFNs), transcription activator-like effector nuclease (TALENs), and clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system, is a promising approach for the treatment of… read more here.
Keywords: crispr cas9; mito crispr; cas9; cas9 system ... See more keywords
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-18548-9
Abstract: The CRISPR-Cas9 system has increased the speed and precision of genetic editing in cells and animals. However, model generation for drug development is still expensive and time-consuming, demanding more target flexibility and faster turnaround times… read more here.
Keywords: obligare doxycycline; doxycycline inducible; cas9 system; doxycycline ... See more keywords