Genome Editing in Mice Using CRISPR/Cas9 Technology
Sign Up to like & getrecommendations! Published in 2018 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.57
Abstract: CRISPR/Cas9 technology has revolutionized genome editing in mice, allowing for simple and rapid development of knockouts and knockins. CRISPR relies on small guide RNAs that direct the RNA‐guided nuclease Cas9 to a designated genomic site… read more here.
Keywords: genome editing; crispr cas9; cas9 technology; cas9 ... See more keywords
The Use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7896-0_27
Abstract: The CRISPR-Cas9 technology is a powerful tool that enables site-specific genome modification (gene editing) and is increasingly used in research to generate gene knockout or knock-in in a variety of cells and organisms. This chapter… read more here.
Keywords: crispr cas9; airway biology; biology; human airway ... See more keywords
CRIPSR/Cas9 Technology: Hypoimmunogenic Pluripotent Stem Cells Evade Immune Rejection in Fully Immunocompetent Allogeneic Recipients
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Heart and Lung Transplantation"
DOI: 10.1016/j.healun.2021.01.1806
Abstract: Purpose Induced pluripotent stem cells (iPSCs) are promising candidates for regenerative medicine. To overcome the allogeneic immune barrier, we created hypo-immune iPSCs using CRISPR/Cas9 editing strategies and demonstrate long-term survival of allografts without the need… read more here.
Keywords: pluripotent stem; ipscs; cas9 technology; stem cells ... See more keywords
Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology.
Sign Up to like & getrecommendations! Published in 2019 at "Stem cell research"
DOI: 10.1016/j.scr.2019.101667
Abstract: Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent inherited renal disease, characterized by multiple cysts that can lead to kidney failure resulting in end-stage renal disease. ADPKD is mainly caused by mutations in… read more here.
Keywords: two isogenic; crispr cas9; pkd2; cas9 technology ... See more keywords
State‐of‐the‐art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Eukaryotic Microbiology"
DOI: 10.1111/jeu.12747
Abstract: CRISPR/Cas9 technology has revolutionized biology. This prokaryotic defense system against foreign DNA has been repurposed for genome editing in a broad range of cell tissues and organisms. Trypanosomatids are flagellated protozoa belonging to the order… read more here.
Keywords: crispr cas9; biology; cas9 technology; genome editing ... See more keywords
The Impact of CRISPR/Cas9 Technology on Cardiac Research: From Disease Modelling to Therapeutic Approaches
Sign Up to like & getrecommendations! Published in 2017 at "Stem Cells International"
DOI: 10.1155/2017/8960236
Abstract: Genome-editing technology has emerged as a powerful method that enables the generation of genetically modified cells and organisms necessary to elucidate gene function and mechanisms of human diseases. The clustered regularly interspaced short palindromic repeats-… read more here.
Keywords: genome editing; research; crispr cas9; cas9 technology ... See more keywords
Inhibition of Hepatitis B Virus with the Help of CRISPR/Cas9 Technology.
Sign Up to like & getrecommendations! Published in 2020 at "Critical reviews in eukaryotic gene expression"
DOI: 10.1615/critreveukaryotgeneexpr.2020028453
Abstract: Hepatitis B infection caused by hepatitis B virus (HBV) is a serious health issue worldwide. Existing therapeutic strategies hardly eradicate HBV infections, and they fail to attain complete cure. Advanced treatment strategies are urgently needed… read more here.
Keywords: hepatitis; crispr cas9; cas9 technology; hepatitis virus ... See more keywords