Development of a yeast heterologous expression cassette based on the promoter and terminator elements of the Eremothecium cymbalariae translational elongation factor 1α (EcTEF1) gene
Sign Up to like & getrecommendations! Published in 2018 at "3 Biotech"
DOI: 10.1007/s13205-018-1224-0
Abstract: A new expression cassette (EC0) consisting of the fused 5′ and 3′ intergenic regions (IGRs) of the Eremothecium cymbalariae translational elongation factor 1α (EcTEF1) gene was evaluated through expression of the bacterial hygromycin B phosphotransferase… read more here.
Keywords: cassette; hygromycin; gene; expression cassette ... See more keywords
Effect of neutral leaks on pumping efficiency in 3D DEMO divertor configuration
Sign Up to like & getrecommendations! Published in 2018 at "Fusion Engineering and Design"
DOI: 10.1016/j.fusengdes.2018.04.089
Abstract: Abstract In this paper we analyze the pumping efficiency of a generic 3D DEMO divertor configuration based on the size of inter-cassette gaps. Due to symmetry conditions we consider only one segment, which is composed… read more here.
Keywords: demo divertor; cassette; pumping efficiency; divertor ... See more keywords
Thermal-hydraulic study of the DEMO divertor cassette body cooling circuit equipped with a liner and two reflector plates
Sign Up to like & getrecommendations! Published in 2021 at "Fusion Engineering and Design"
DOI: 10.1016/j.fusengdes.2021.112227
Abstract: Abstract In the framework of the Work Package DIV 1 – “Divertor Cassette Design and Integration” of the EUROfusion action, a research campaign has been jointly carried out by University of Palermo and ENEA to… read more here.
Keywords: divertor cassette; cassette body; cassette; demo divertor ... See more keywords
An array of multiplex PCR assays for detection of staphylococcal chromosomal cassette mec (SCCmec) types among staphylococcal isolates.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2019.105733
Abstract: Staphylococcal Cassette Chromosome mec (SCCmec) element plays a key role in development of resistance by carrying different resistance factors. Therefore, routine and rapid diagnostic approach is considered advantageous for the easy detection of SCCmec elements.… read more here.
Keywords: type type; cassette; multiplex pcr; type ... See more keywords
Bacterial Genome Editing via a Designed Toxin-Antitoxin Cassette.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.6b00287
Abstract: Manipulating the bacterial genomes in an efficient manner is essential to biological and biotechnological research. Here, we reprogrammed the bacterial TA systems as the toxin counter-selectable cassette regulated by an antitoxin switch (TCCRAS) for genetic… read more here.
Keywords: genome editing; cassette; bacterial genome; editing via ... See more keywords
Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-00400-2
Abstract: Sensitivity, dynamic and detection range as well as exclusion of expression and instrumental artifacts are critical for the quantitation of data obtained with fluorescent protein (FP)-based biosensors in vivo. Current biosensors designs are, in general,… read more here.
Keywords: green orange; cassette; ratiometric matryoshka; fluorescent protein ... See more keywords
Casboundary: automated definition of integral Cas cassettes
Sign Up to like & getrecommendations! Published in 2021 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btaa984
Abstract: Abstract Motivation CRISPR-Cas are important systems found in most archaeal and many bacterial genomes, providing adaptive immunity against mobile genetic elements in prokaryotes. The CRISPR-Cas systems are encoded by a set of consecutive cas genes,… read more here.
Keywords: casboundary automated; automated definition; cas genes; cassette ... See more keywords
Cassette recruitment in the chromosomal Integron of Vibrio cholerae
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab412
Abstract: Abstract Integrons confer a rapid adaptation capability to bacteria. Integron integrases are able to capture and shuffle novel functions embedded in cassettes. Here, we investigated cassette recruitment in the Vibrio cholerae chromosomal integron during horizontal… read more here.
Keywords: chromosomal integron; integron; cassette; cholerae ... See more keywords
Alternative Splicing in the Nuclear Receptor Superfamily Expands Gene Function to Refine Endo-Xenobiotic Metabolism
Sign Up to like & getrecommendations! Published in 2020 at "Drug Metabolism and Disposition"
DOI: 10.1124/dmd.119.089102
Abstract: The human genome encodes 48 nuclear receptor (NR) genes, whose translated products transform chemical signals from endo-xenobiotics into pleotropic RNA transcriptional profiles that refine drug metabolism. This review describes the remarkable diversification of the 48… read more here.
Keywords: methodology; metabolism; cassette; endo xenobiotic ... See more keywords
Characterization of SCCmec Instability in Methicillin-Resistant Staphylococcus aureus Affecting Adjacent Chromosomal Regions, Including the Gene for Staphylococcal Protein A (spa)
Sign Up to like & getrecommendations! Published in 2022 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.02374-21
Abstract: Staphylococcal cassette chromosome mec (SCCmec) represents a sequence of clear clinical and diagnostic importance in staphylococci. At a minimum the chromosomal cassette contains the mecA gene encoding PBP2a but frequently also includes additional antibiotic resistance… read more here.
Keywords: cassette; staphylococcal protein; methicillin resistant; sccmec ... See more keywords
Dissecting the invasion of Galleria mellonella by Yersinia enterocolitica reveals metabolic adaptations and a role of a phage lysis cassette in insect killing
Sign Up to like & getrecommendations! Published in 2022 at "PLOS Pathogens"
DOI: 10.1371/journal.ppat.1010991
Abstract: The human pathogen Yersinia enterocolitica strain W22703 is characterized by its toxicity towards invertebrates that requires the insecticidal toxin complex (Tc) proteins encoded by the pathogenicity island Tc-PAIYe. Molecular and pathophysiological details of insect larvae… read more here.
Keywords: cassette; yersinia enterocolitica; invasion; galleria mellonella ... See more keywords