Rational designed mutagenesis of levansucrase from Bacillus licheniformis 8-37-0-1 for product specificity study
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-8854-3
Abstract: Levansucrases, which belong to the glycoside hydrolase family 68 (GH68), synthesize β (2-6)-linked fructan levan with sucrose as substrate. We described the use of a levansucrase (Bl_SacB) from Bacillus licheniformis 8-37-0-1 for catalysis of fructosyl… read more here.
Keywords: bacillus licheniformis; product; catalytic triad; fru glc ... See more keywords
Activity modulation of the oligopeptidase B from Serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine.
Sign Up to like & getrecommendations! Published in 2017 at "Biochimie"
DOI: 10.1016/j.biochi.2017.05.013
Abstract: Oligopeptidase B (OpdB; EC 3.4.21.83) is a trypsin-like peptidase belonging to the family of serine prolyl oligopeptidases; two-domain structure of the enzyme includes C-terminal peptidase catalytic domain and N-terminal seven-bladed β-propeller domain. Importance of the… read more here.
Keywords: serratia proteamaculans; amino acid; surrounding catalytic; catalytic triad ... See more keywords
Enzyme inspired polymer functionalized with an artificial catalytic triad
Sign Up to like & getrecommendations! Published in 2021 at "Polymer"
DOI: 10.1016/j.polymer.2021.123735
Abstract: Abstract At the heart of an enzyme’s structure is the active catalytic site, which, together with the surrounding amino-acid residues, tunes the substrate properties in the electrostatic microenvironment. Taking inspiration from nature’s catalytic triad, consisting… read more here.
Keywords: enzyme inspired; artificial catalytic; inspired polymer; catalytic triad ... See more keywords
Spatial Coordination of Cooperativity in Silica-Supported Cu/TEMPO/Imidazole Catalytic Triad
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DOI: 10.1021/acscatal.8b01399
Abstract: Multifunctional catalysts obtained by the covalent immobilization of discrete molecular species on porous supports represent a unique approach to emulate some of the design principle and performances of enzymes. However, it is decisive in such… read more here.
Keywords: coordination cooperativity; spatial coordination; silica; catalytic triad ... See more keywords
DWV 3C Protease Uncovers the Diverse Catalytic Triad in Insect RNA Viruses
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00068-22
Abstract: The number of managed honey bee (Apis mellifera) colonies has considerably declined in many developed countries in the recent years. Deformed wing virus (DWV) vectored by the mites is the major threat to honey bee… read more here.
Keywords: insect rna; protease; honey; dwv ... See more keywords