Rational design of disulfide bonds to increase thermostability of Rhodococcus opacus catechol 1,2 dioxygenase
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28808
Abstract: Catechol 1,2 dioxygenase is a versatile enzyme with several potential applications. However, due to its low thermostability, its industrial potential is not being met. In this study, the thermostability of a mesophilic catechol 1,2 dioxygenase… read more here.
Keywords: rhodococcus opacus; design disulfide; thermostability; catechol dioxygenase ... See more keywords
Catechol 2,3‐dioxygenase from a new phenolic compound degrader Thauera sp. K11: purification and biochemical characterization
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201700566
Abstract: Catechol 2,3‐dioxygenase (C23O) from a new phenolic compound degrader Thauera sp. K11 was purified and characterized. The native form of the enzyme was determined as a homotetramer with a molecular weight of 140 kDa, and its… read more here.
Keywords: catechol dioxygenase; new phenolic; phenolic compound; degrader thauera ... See more keywords
A Novel Cold‐Adapted Catechol 1,2‐Dioxygenase From Antarctic Sea‐Ice Bacterium Halomonas sp. ANT108: Characterization and Immobilization
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.202400500
Abstract: The enzyme catechol 1,2‐dioxygenase (CAT) plays a critical role in the biosynthesis pathway of cis, cis‐muconic acid (CCMA), which serves as an indispensable raw material for various industrial applications. In this research, we cloned a… read more here.
Keywords: novel cold; antarctic sea; sea ice; ice bacterium ... See more keywords
Phenol degradation and genotypic analysis of dioxygenase genes in bacteria isolated from sediments
Sign Up to like & getrecommendations! Published in 2017 at "Brazilian Journal of Microbiology"
DOI: 10.1016/j.bjm.2016.12.002
Abstract: The aerobic degradation of aromatic compounds by bacteria is performed by dioxygenases. To show some characteristic patterns of the dioxygenase genotype and its degradation specificities, twenty-nine gram-negative bacterial cultures were obtained from sediment contaminated with… read more here.
Keywords: degradation; dioxygenase; catechol dioxygenase; dioxygenase gene ... See more keywords
Expression and characterization of catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3.
Sign Up to like & getrecommendations! Published in 2021 at "Protein expression and purification"
DOI: 10.1016/j.pep.2021.105964
Abstract: The gene of catechol 1, 2-dioxygenase was identified and cloned from the genome of Oceanimonas marisflavi 102-Na3. The protein was expressed in Escherichia coli BL21 (DE3) and purified to homogeneity of a dimer with molecular… read more here.
Keywords: oceanimonas marisflavi; catechol dioxygenase; 102 na3; marisflavi 102 ... See more keywords
A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-03144-8
Abstract: Identification of novel enzymes from lignin degrading microorganisms will help to develop biotechnologies for biomass valorization and aromatic hydrocarbons degradation. Bacillus ligniniphilus L1 grows with alkaline lignin as the single carbon source and is a… read more here.
Keywords: ligniniphilus catechol; catechol; bacillus ligniniphilus; catechol dioxygenase ... See more keywords
Investigating the quaternary structure of a homomultimeric catechol 1,2-dioxygenase: An integrative structural biology study
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DOI: 10.1371/journal.pone.0315992
Abstract: The structural analysis of catechol 1,2 dioxygenase from Stutzerimonas frequens GOM2, SfC12DO, was conducted using various structural techniques. SEC-SAXS experiments revealed that SfC12DO, after lyophilization and reconstitution processes, can form multiple enzymatically active oligomers, including… read more here.
Keywords: quaternary structure; investigating quaternary; biology; catechol dioxygenase ... See more keywords
Characterization of Catechol-1,2-Dioxygenase (Acdo1p) From Blastobotrys raffinosifermentans and Investigation of Its Role in the Catabolism of Aromatic Compounds
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.872298
Abstract: Gallic acid, protocatechuic acid, catechol, and pyrogallol are only a few examples of industrially relevant aromatics. Today much attention is paid to the development of new microbial factories for the environmentally friendly biosynthesis of industrially… read more here.
Keywords: dioxygenase acdo1p; blastobotrys raffinosifermentans; acid; catechol dioxygenase ... See more keywords