Strategies for Converting RNA to Amplifiable cDNA for Single-Cell RNA Sequencing Methods.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-13-6037-4_1
Abstract: This review describes the features of molecular biology techniques for single-cell RNA sequencing (scRNA-seq), including methods developed in our laboratory. Existing scRNA-seq methods require the conversion of first-strand cDNA to amplifiable cDNA followed by whole-transcript… read more here.
Keywords: single cell; amplifiable cdna; rna; biology ... See more keywords
Purification and cDNA Cloning of Antimicrobial Peptides from the Skin Secretion of the Chinese Frog Rana chensinensis
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Peptide Research and Therapeutics"
DOI: 10.1007/s10989-020-10074-y
Abstract: The skin secretions of amphibians are a rich source of bioactive peptides. We isolated chensirin-1 and chensirin-2 from the skin secretion of the Chinese frog Rana chensinensis . Sephadex-G-50 and RP-HPLC were employed to purify… read more here.
Keywords: secretion chinese; rana chensinensis; frog rana; chinese frog ... See more keywords
Complement factor C5 in Atlantic salmon (Salmo salar): Characterization of cDNA, protein and glycosylation.
Sign Up to like & getrecommendations! Published in 2019 at "Developmental and comparative immunology"
DOI: 10.1016/j.dci.2019.103424
Abstract: Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates. We report the characterization of C5 cDNA and protein from Atlantic salmon (Salmo salar), a teleost fish species of… read more here.
Keywords: salmon salmo; characterization cdna; cdna protein; salmo salar ... See more keywords
A novel fluorescent enhancing platform based on DNA-scaffolded silver nanoclusters for potential inflammatory bowel disease-associated microRNA detection.
Sign Up to like & getrecommendations! Published in 2020 at "Talanta"
DOI: 10.1016/j.talanta.2020.121122
Abstract: DNA-scaffolded silver nanoclusters (DNA/AgNC) probes are widely used to detect microRNAs (miRNAs) for diagnosing diseases. However, current available DNA/AgNC probes, which primarily based on fluorescence quenching (turn-off) method, suffer from low detection accuracy caused by… read more here.
Keywords: fluorescence; dna; dna scaffolded; dna agnc ... See more keywords
A bivalent binding aptamer-cDNA on MoS2 nanosheets based fluorescent aptasensor for detection of aflatoxin M1.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122779
Abstract: To ensure the safety of dairy products, especially milk, and consequently protect human health, accurate and simple analytical techniques are highly necessary to determine the low concentration of aflatoxin M1 (AFM1) as an important carcinogen.… read more here.
Keywords: detection; bivalent binding; binding aptamer; fluorescent aptasensor ... See more keywords
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-23779-5
Abstract: The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA genome (~30 kb), infectious full-length cDNA is readily assembled in… read more here.
Keywords: genetics; rna; versatile reverse; sars cov ... See more keywords
Coronavirus genomic cDNA derived G-quadruplex as a selective target for fluorometric detection.
Sign Up to like & getrecommendations! Published in 2023 at "Chemical communications"
DOI: 10.1039/d3cc00740e
Abstract: Pathogenic genomes harboring noncanonical G-quadruplex (GQ) forming sequences are potential targets for diagnosis. The GQ-forming cDNA sequences of SARS-CoV-2 (Severe acute respiratory syndrome coronavirus-2) are identified and validated as reliable diagnostic targets. The high fidelity… read more here.
Keywords: genomic cdna; coronavirus genomic; detection; derived quadruplex ... See more keywords
Sensitive and accurate analysis of gene expression signatures enabled by oligonucleotide-labelled cDNA
Sign Up to like & getrecommendations! Published in 2022 at "RNA Biology"
DOI: 10.1080/15476286.2022.2078093
Abstract: ABSTRACT High-throughput RNA sequencing offers a comprehensive analysis of transcriptome complexity originated from regulatory events, such as differential gene expression, alternative polyadenylation and others, and allows the increase in diagnostic capacity and precision. For gene… read more here.
Keywords: analysis; sensitive accurate; gene expression; gene ... See more keywords
Meiotic cDNA Libraries Reveal Gene Truncations and Mitochondrial Proteins Important for Competitive Fitness in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2022 at "Genetics"
DOI: 10.1093/genetics/iyac066
Abstract: Gametogenesis is an evolutionarily conserved developmental program whereby a diploid progenitor cell undergoes meiosis and cellular remodeling to differentiate into haploid gametes, the precursors for sexual reproduction. Even in the simple eukaryotic organism Saccharomyces cerevisiae,… read more here.
Keywords: cdna libraries; gene truncations; cdna; competitive fitness ... See more keywords
RNA-Seq: RNA Conversion to cDNA and Amplification.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot094672
Abstract: The Ovation RNA-Seq Kit provides a fast and simple method for preparing amplified cDNA from total RNA. Amplification is initiated both at the 3' ends of the transcripts and across the entire range of transcribed… read more here.
Keywords: rna conversion; seq rna; rna; cdna ... See more keywords
Long-Read Annotation: Automated Eukaryotic Genome Annotation Based on Long-Read cDNA Sequencing1[OPEN]
Sign Up to like & getrecommendations! Published in 2018 at "Plant Physiology"
DOI: 10.1104/pp.18.00848
Abstract: The long Read Annotation (LoReAn) pipeline provides automated genome annotation with superior performance by incorporating single-molecule cDNA sequencing data. Single-molecule full-length complementary DNA (cDNA) sequencing can aid genome annotation by revealing transcript structure and alternative… read more here.
Keywords: long read; annotation; read annotation; cdna ... See more keywords