Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing libraries.
Sign Up to like & getrecommendations! Published in 2019 at "BioTechniques"
DOI: 10.2144/btn-2018-0178
Abstract: RNA sequencing library construction using single-stranded ligation of a DNA adapter to 3' ends of cDNAs often produces primer-adapter byproducts, which compete with cDNA-adapter ligation products during library amplification and, therefore, reduces the number of… read more here.
Keywords: cdna ligation; based sequencing; ligation; ligation based ... See more keywords