Template-switching artifacts resemble alternative polyadenylation
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-6199-7
Abstract: BackgroundAlternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally disregarded, while alternative polyadenylation artifacts are… read more here.
Keywords: template switching; switching artifacts; polyadenylation; cdna sequencing ... See more keywords
Native RNA or cDNA Sequencing for Transcriptomic Analysis: A Case Study on Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.842299
Abstract: Direct sequencing of single molecules through nanopores allows for accurate quantification and full-length characterization of native RNA or complementary DNA (cDNA) without amplification. Both nanopore-based native RNA and cDNA approaches involve complex transcriptome procedures at… read more here.
Keywords: cdna sequencing; rna sequencing; rna; cdna ... See more keywords
Improved Nanopore full-length cDNA sequencing by PCR-suppression
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.1031355
Abstract: Full-length transcript sequencing remains a main goal of RNA sequencing. However, even the application of long-read sequencing technologies such as Oxford Nanopore Technologies still fail to yield full-length transcript sequencing for a significant portion of… read more here.
Keywords: full length; length cdna; cdna sequencing; pcr suppression ... See more keywords
cDNA sequencing increases the molecular diagnostic yield in Chediak-Higashi syndrome
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2023.1072784
Abstract: Introduction: Chediak-Higashi syndrome (CHS) is rare autosomal recessive disorder caused by bi-allelic variants in the Lysosomal Trafficking Regulator (LYST) gene. Diagnosis is established by the detection of pathogenic variants in LYST in combination with clinical… read more here.
Keywords: molecular diagnostic; cdna sequencing; sanger sequencing; sanger ... See more keywords