Use of an intron containing grapevine gene as internal control for validation of cDNA synthesis in virus detection by RT-PCR
Sign Up to like & getrecommendations! Published in 2017 at "European Journal of Plant Pathology"
DOI: 10.1007/s10658-017-1218-5
Abstract: Detection of viruses in grapevine plants is important to prevent their spreading with propagating stocks. For this purpose PCR based protocols are commonly and routinely used. Since most grapevine viruses are RNA viruses, the detection… read more here.
Keywords: internal control; detection; virus detection; cdna synthesis ... See more keywords
A novel role of TRIM28 B box domain in L1 retrotransposition and ORF2p-mediated cDNA synthesis
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad247
Abstract: Abstract The long interspersed element 1 (LINE-1 or L1) integration is affected by many cellular factors through various mechanisms. Some of these factors are required for L1 amplification, while others either suppress or enhance specific… read more here.
Keywords: box domain; retrotransposition; cdna synthesis; trim28 box ... See more keywords
Increasing the Sensitivity of Leishmania RNA Virus 2 (LRV2) Detection with a Modification in cDNA Synthesis.
Sign Up to like & getrecommendations! Published in 2022 at "Turkiye parazitolojii dergisi"
DOI: 10.4274/tpd.galenos.2022.30074
Abstract: OBJECTIVE Leishmania RNA virus was detected the first time in the New World Leishmania species. Recent studies were also showed the presence of Leishmania RNA virus 2 (LRV2) in Old Word Leishmania species including Turkish… read more here.
Keywords: leishmania rna; sensitivity; cdna synthesis; leishmania ... See more keywords