Reporter cell assay-based functional quantification of TNF-α-antagonists in serum -- a proof-of-principle study for adalimumab.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113646
Abstract: Quantification of therapeutic antibodies is commonly based on physico-chemical assays such as enzyme-linked immunoabsorption assays (ELISA) and lately on mass spectrometry. However, the functional integrity of evaluated immunoglobulins is yet not assessed. Consequently, a commercially… read more here.
Keywords: cell assay; quantification; reporter cell; functional quantification ... See more keywords
FORTIS: a live-cell assay to monitor AMPA receptors using pH-sensitive fluorescence tags
Sign Up to like & getrecommendations! Published in 2021 at "Translational Psychiatry"
DOI: 10.1038/s41398-021-01457-w
Abstract: The real-time live fluorescent monitoring of surface AMPA receptors (AMPARs) could open new opportunities for drug discovery and phenotypic screening concerning neuropsychiatric disorders. We have developed FORTIS, a tool based on pH sensitivity capable of… read more here.
Keywords: cell assay; live cell; surface ampars; fortis live ... See more keywords
Use of the microfluidic impedance red cell assay in sickle cell disease
Sign Up to like & getrecommendations! Published in 2025 at "Blood Advances"
DOI: 10.1182/bloodadvances.2024015590
Abstract: Key Points • The MIRCA is a reproducible assay with readouts that correlate to SCD severity.• We propose using MIRCA to measure RBC deformability at clinic visits to monitor SCD complication risk and need for… read more here.
Keywords: impedance red; use microfluidic; microfluidic impedance; cell ... See more keywords
Immunogenicity Risk Assessment of Biotherapeutics Using an Ex Vivo B Cell Assay
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DOI: 10.3390/antib14030062
Abstract: Background/Objectives: Anti-drug antibody (ADA) formation can impact the safety, pharmacokinetics, and/or efficacy of biotherapeutics, including monoclonal antibodies (mAbs). Current strategies for ADA/immunogenicity risk prediction of mAbs include in silico algorithms, T cell proliferation assays, MHC-associated… read more here.
Keywords: risk prediction; immunogenicity risk; cell; cell assay ... See more keywords
Prognostic Relevance of Multi-Antigenic Myeloma-Specific T-Cell Assay in Patients with Monoclonal Gammopathies
Sign Up to like & getrecommendations! Published in 2023 at "Cancers"
DOI: 10.3390/cancers15030972
Abstract: Simple Summary Multiple myeloma (MM) is a hematologic cancer originating from underlying precursor disorders, called Monoclonal Gammopathy of Undetermined Significance (MGUS) and Smoldering Multiple Myeloma (SMM). In order to predict the risk of progression from… read more here.
Keywords: myeloma specific; specific cell; myeloma; antigenic myeloma ... See more keywords