PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing
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DOI: 10.1039/d1sc05558e
Abstract: The CRISPR-Cas system has been repurposed as a powerful live-cell imaging tool, but its utility is limited to genomic loci and mRNA imaging in living cells. Here, we demonstrated the potential of the CRISPR-Cas system… read more here.
Keywords: system; crispr cas12a; cell biosensing; dna substrates ... See more keywords
MnO2 nanosheets as a carrier and accelerator for improved live-cell biosensing application of CRISPR/Cas12a
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DOI: 10.1039/d1sc06383a
Abstract: Besides gene-editing, the CRISPR/Cas12a system has also been widely used in in vitro biosensing, but its applications in live-cell biosensing are rare. One reason is lacking appropriate carriers to synchronously deliver all components of the… read more here.
Keywords: cell biosensing; crispr cas12a; live cell; mno2 nanosheets ... See more keywords