Achieving greater efficiency and higher confidence in single‐cell cloning by combining cell printing and plate imaging technologies
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2698
Abstract: In recent years, health authorities have increased emphasis on demonstrating that a cell line, which is used for the generation of biologics, is clonally derived. Within the past few years, single‐cell manipulation technologies, especially microfluidic… read more here.
Keywords: single cell; cell cloning; plate imaging; cell printing ... See more keywords
Genetic screens in isogenic mammalian cell lines without single cell cloning
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-14620-6
Abstract: Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for understanding gene function. Generating such pairs of mammalian cells, however, is labor-intensive, time-consuming, and, in some cell types, essentially… read more here.
Keywords: single cell; cell cloning; cell lines; genetic screens ... See more keywords
Single cell cloning generates lung endothelial colonies with conserved growth, angiogenic, and bioenergetic characteristics
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DOI: 10.1177/2045893217731295
Abstract: Pulmonary artery, capillary, and vein endothelial cells possess distinctive structures and functions, which represent a form of vascular segment specific macroheterogeneity. However, within each of these segmental populations, individual cell functional variability represents a poorly… read more here.
Keywords: single cell; cell cloning; growth angiogenic; generation clones ... See more keywords
Generation of Monogenetic Cattle by Different Techniques of Embryonic Cell and Somatic Cell Cloning – Their Application to Biotechnological, Agricultural, Nutritional, Biomedical and Transgenic Research – A Review
Sign Up to like & getrecommendations! Published in 2020 at "Annals of Animal Science"
DOI: 10.2478/aoas-2020-0096
Abstract: Abstract The development of effective approaches for not only the in vitro maturation (IVM) of heifer/cow oocytes and their extracorporeal fertilization (IVF) but also the non-surgical collection and transfer of bovine embryos has given rise… read more here.
Keywords: embryonic cell; cell cloning; somatic cell; transgenic research ... See more keywords