In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer
Sign Up to like & getrecommendations! Published in 2021 at "Cell"
DOI: 10.1016/j.cell.2021.02.019
Abstract: Improving effector activity of antigen-specific T cells is a major goal in cancer immunotherapy. Despite the identification of several effector T cell (TEFF)-driving transcription factors (TFs), the transcriptional coordination of TEFF biology remains poorly understood. We developed… read more here.
Keywords: cd8; cell crispr; biology; cancer ... See more keywords
Model-based understanding of single-cell CRISPR screening
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10216-x
Abstract: The recently developed single-cell CRISPR screening techniques, independently termed Perturb-Seq, CRISP-seq, or CROP-seq, combine pooled CRISPR screening with single-cell RNA-seq to investigate functional CRISPR screening in a single-cell granularity. Here, we present MUSIC, an integrated… read more here.
Keywords: single cell; model based; cell crispr; crispr screening ... See more keywords
SCREE: a comprehensive pipeline for single-cell multi-modal CRISPR screen data processing and analysis.
Sign Up to like & getrecommendations! Published in 2023 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbad123
Abstract: Single-cell CRISPR screens have been widely used to investigate gene regulatory circuits in diverse biological systems. The recent development of single-cell CRISPR screens has enabled multimodal profiling of perturbed cells with both gene expression, chromatin… read more here.
Keywords: cell; cell crispr; crispr screens; single cell ... See more keywords
Abstract 4432: scMAGeCK links genotypes with multiple phenotypes in single-cell CRISPR screens
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Research"
DOI: 10.1158/1538-7445.am2020-4432
Abstract: We present scMAGeCK, a computational framework to identify genomic elements associated with multiple expression-based phenotypes in CRISPR/Cas9 functional screening that uses single-cell RNA-seq as readout. scMAGeCK outperforms existing methods, identifies genes and enhancers with known… read more here.
Keywords: single cell; cell; cell crispr; scmageck links ... See more keywords