Biofabrication Using Electrochemical Devices and Systems
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Biosystems"
DOI: 10.1002/adbi.201900234
Abstract: Biofabrication is roughly defined as techniques producing complex 2D and 3D tissues and organs from raw materials such as living cells, matrices, biomaterials, and molecules. It is useful for tissue engineering, regenerative medicine, drug screening,… read more here.
Keywords: cell culture; systems biofabrication; devices systems; biofabrication ... See more keywords
Hydrophobic Patterning‐Based 3D Microfluidic Cell Culture Assay
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DOI: 10.1002/adhm.201800122
Abstract: Engineering physiologically relevant in vitro models of human organs remains a fundamental challenge. Despite significant strides made within the field, many promising organ-on-a-chip models fall short in recapitulating cellular interactions with neighboring cell types, surrounding… read more here.
Keywords: cell culture; patterning based; hydrophobic patterning; cell ... See more keywords
Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.
Sign Up to like & getrecommendations! Published in 2022 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202102097
Abstract: Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches. LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons… read more here.
Keywords: culture; bioorthogonally cross; cross linked; cell culture ... See more keywords
Human Protein‐Based Porous Scaffolds as Platforms for Xeno‐Free 3D Cell Culture
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202102383
Abstract: Extracellular matrix and protein‐based biomaterials emerge as attractive sources to produce scaffolds due to their great properties regarding biocompatibility and bioactivity. In addition, there are concerns regarding the use of animal‐derived supplements in cell culture… read more here.
Keywords: culture; cell culture; xeno free; protein based ... See more keywords
Instructive Hydrogels for Primary Tumor Cell Culture: Current Status and Outlook
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DOI: 10.1002/adhm.202102479
Abstract: Primary tumor organoids (PTOs) growth in hydrogels have emerged as an important in vitro model that recapitulates many characteristics of the native tumor tissue, and have important applications in fundamental cancer research and for the… read more here.
Keywords: instructive hydrogels; cell culture; primary tumor; tumor cell ... See more keywords
Recent Advances on cell Culture Platforms for in vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202202936
Abstract: The clinical translations of drugs and nanomedicines depend on coherent pharmaceutical research based on biologically accurate screening approaches. Since establishing the 2D in vitro cell culture method, the scientific community has improved cell-based drug screening… read more here.
Keywords: drug screening; cell culture; cell; based microfluidics ... See more keywords
Electropermeabilization of metastatic chondrosarcoma cells from primary cell culture
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1809
Abstract: Primary cell cultures are challenging, but reliable model reflecting tumor response in vitro. The study was designed to examine if the increased electropermeabilization can overcame initial drug insensitivity in chondrosarcoma cells from lung metastasis. We… read more here.
Keywords: electropermeabilization; cell culture; primary cell; chondrosarcoma cells ... See more keywords
Real‐time amino acid and glucose monitoring system for the automatic control of nutrient feeding in CHO cell culture using Raman spectroscopy
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100395
Abstract: An innovative, Raman spectroscopy‐based monitoring and control system is introduced in this paper for designing dynamic feeding strategies that allow the maintenance of key cellular nutrients at an ideal level in Chinese hamster ovary cell… read more here.
Keywords: cell culture; spectroscopy; control; real time ... See more keywords
High‐efficiency affinity precipitation of multiple industrial mAbs and Fc‐fusion proteins from cell culture harvests using Z‐ELP‐E2 nanocages
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DOI: 10.1002/bit.26717
Abstract: Affinity precipitation using Z‐elastin‐like polypeptide‐functionalized E2 protein nanocages has been shown to be a promising alternative to Protein A chromatography for monoclonal antibody (mAb) purification. We have previously described a high‐yielding, affinity precipitation process capable… read more here.
Keywords: protein; affinity; affinity precipitation; precipitation ... See more keywords
Interaction of cell culture process parameters for modulating mAb afucosylation
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DOI: 10.1002/bit.26908
Abstract: The extent of afucosylation, which refers to the absence of core fucose on Fc glycans, can correlate positively with the antibody‐dependent cellular cytotoxicity (ADCC) activity of a monoclonal antibody (mAb). Therefore, it is important to… read more here.
Keywords: cell culture; culture; culture process; afucosylation ... See more keywords
Vitamins in cell culture media: Stability and stabilization strategies
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26942
Abstract: Nowadays, chemically defined cell culture media (CCM) have replaced serum‐ and hydrolysate‐based media that rely on complex ingredients, such as yeast extracts or peptones. Benefits include a significantly lower lot‐to‐lot variability, more efficient manufacturing by… read more here.
Keywords: stabilization; cell culture; culture media; vitamins cell ... See more keywords