Lactate Detection in Tumor Cell Cultures Using Organic Transistor Circuits.
Sign Up to like & getrecommendations! Published in 2017 at "Advanced materials"
DOI: 10.1002/adma.201605744
Abstract: A biosensing platform based on an organic transistor circuit for metabolite detection in highly complex biological media is introduced. The sensor circuit provides inherent background subtraction allowing for highly specific, sensitive lactate detection in tumor… read more here.
Keywords: detection; organic transistor; lactate detection; detection tumor ... See more keywords
Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology journal"
DOI: 10.1002/biot.201700425
Abstract: Oxidative stress that naturally accumulates in the endoplasmic reticulum (ER) as a result of mitochondrial energy metabolism and protein synthesis can disturb the ER function. Because ER have a responsibility on the protein synthesis and… read more here.
Keywords: baicalein; antibody; cell cultures; cell ... See more keywords
Establishment of 2D Cell Cultures Derived From 3D MCF-7 Spheroids Displaying a Doxorubicin Resistant Profile.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology journal"
DOI: 10.1002/biot.201800268
Abstract: In vitro 3D cancer spheroids generally exhibit a drug resistance profile similar to that found in solid tumors. Due to this property, these models are an appealing for anticancer compounds screening. Nevertheless, the techniques and… read more here.
Keywords: derived mcf; resistant profile; resistance; cell cultures ... See more keywords
Blockage of undesirable endocytosis of recombinant human growth/differentiation factor‐5 in Chinese hamster ovary cell cultures requires heparin analogs with specific chain lengths
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology Journal"
DOI: 10.1002/biot.202100227
Abstract: Cell surface heparan sulfate proteoglycan (HSPG)‐mediated endocytosis lowers the yield of recombinant human bone morphogenetic proteins (rhBMPs), such as rhBMP‐2 and rhBMP‐4, from Chinese hamster ovary (CHO) cell cultures. Exogenous recombinant human growth/differentiation factor‐5 (rhGDF‐5),… read more here.
Keywords: chain; recombinant human; rhgdf internalization; cell cultures ... See more keywords
Whole‐cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26254
Abstract: Many high‐value added recombinant proteins, such as therapeutic glycoproteins, are produced using mammalian cell cultures. In order to optimize the productivity of these cultures it is important to monitor cellular metabolism, for example the utilization… read more here.
Keywords: whole cell; biosensor; mammalian cell; lactate ... See more keywords
Probabilistic model by Bayesian network for the prediction of antibody glycosylation in perfusion and fed‐batch cell cultures
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27769
Abstract: Glycosylation is a critical quality attribute of therapeutic monoclonal antibodies (mAbs). The glycan pattern can have a large impact on the immunological functions, serum half‐life and stability. The medium components and cultivation parameters are known… read more here.
Keywords: network; bayesian network; glycosylation; perfusion fed ... See more keywords
Modulating and optimizing Pluronic F-68 concentrations and feeding for intensified perfusion Chinese hamster ovary cell cultures.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3340
Abstract: Perfusion culture is often performed with micro-sparger to fulfill the high oxygen demand from the densified cells. Protective additive Pluronic F-68 (PF-68) is widely used to mitigate the adverse effect in cell viability from micro-sparging.… read more here.
Keywords: cell; chinese hamster; hamster ovary; cell cultures ... See more keywords
Imaging of dense cell cultures by multiwavelength lens‐free video microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23079
Abstract: They present results for lens‐free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction… read more here.
Keywords: microscopy; dense cell; lens free; cell cultures ... See more keywords
Cancer Cytokines and the Relevance of 3D Cultures for Studying Those Implicated in Human Cancers
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DOI: 10.1002/jcb.25970
Abstract: Cancers are complex conditions and involve several factors for oncogenesis and progression. Of the various factors influencing the physiology of cancers, cytokines are known to play significant roles as mediators of functions. Intricate cytokine networks… read more here.
Keywords: research; cancer cytokines; cultures studying; cancer ... See more keywords
Hematopoietic Stem Cell Cultures and Assays.
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DOI: 10.1007/978-1-0716-1028-2_30
Abstract: The adult hematopoietic system is repopulated in its entirety from a rare cell type known as hematopoietic stem cells (HSCs) that reside in the marrow space throughout the skeletal system. Here we describe the isolation… read more here.
Keywords: cultures assays; stem cell; hematopoietic stem; cell cultures ... See more keywords
LC-MS/MS-Based Metabolomics for Cell Cultures.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9477-9_10
Abstract: Metabolomics, a comprehensive analysis of metabolites in biological specimens (e.g., cells, body fluids, tissues, exhaled air, plants), offers promising tools in health, nutrition, biotechnology, and food sciences. Here we describe methods of LC-MS/MS-based analyses for… read more here.
Keywords: analysis; biology; metabolomics cell; based metabolomics ... See more keywords