Evaluation on H2O2-aided ultrasonic pretreatment for cell disruption of Chlorella pyrenoidosa
Sign Up to like & getrecommendations! Published in 2017 at "Asia-Pacific Journal of Chemical Engineering"
DOI: 10.1002/apj.2093
Abstract: In this work, Chlorella pyrenoidosa was chosen as model microalgae, and several pretreatments, including hydroxyl-peroxide-aided ultrasonic pretreatments and mono-ultrasonication (US), were used to rupture the microalgal cells for extracting cellular inclusion. The disruption efficiency and… read more here.
Keywords: disruption; aided ultrasonic; cell disruption; cell ... See more keywords
High recovery of intracellular recombinant HBsAg from Pichia pastoris via continuous pulsed laser cell disruption system optimized by response surface methodology
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1701
Abstract: In our previous study, we demonstrated that continuous power laser could be a clean, rapid, and convenient alternative to the other conventional disruption techniques for the release of recombinant hepatitis B surface antigen (rHBsAg) from… read more here.
Keywords: pulsed laser; methodology; laser; cell disruption ... See more keywords
Microscale disruption of microorganisms for parallelized process development
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Journal"
DOI: 10.1002/biot.201600579
Abstract: Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris are the standard platforms for biopharmaceutical production with 40% of all between 2010 to 2014 approved protein drugs produced in those microbial hosts. Typically, products overexpressed E. coli… read more here.
Keywords: process development; cell disruption; disruption; microscale ... See more keywords
Optimization of Cell Disruption and Transesterification of Lipids from Botryococcus braunii LB572
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-018-0277-6
Abstract: Several methods including microwave, Frenchpress, autoclave, bead-beating, ultrasonication, and osmotic shock were compared to identify the most effective microalgal cell disruption method. Botryococcus braunii LB572 was cultured in 5 L flasks containing JM medium mixed… read more here.
Keywords: braunii lb572; transesterification; botryococcus braunii; cell disruption ... See more keywords
Thermo-responsive switchable solvents for simultaneous microalgae cell disruption, oil extraction-reaction, and product separation for biodiesel production
Sign Up to like & getrecommendations! Published in 2020 at "Biocatalysis and agricultural biotechnology"
DOI: 10.1016/j.bcab.2020.101667
Abstract: Abstract A thermo-responsive switchable solvent (TSS), which switched hydrophobicity by the change in temperature, was successfully used for simultaneous cell disruption, oil extraction-reaction, and biodiesel separation from wet microalgae. Catalyzed by immobilized lipase, the entire… read more here.
Keywords: biodiesel; biodiesel production; reaction; cell disruption ... See more keywords
Mechanical cell disruption of Parachlorella kessleri microalgae: Impact on lipid fraction composition.
Sign Up to like & getrecommendations! Published in 2018 at "Bioresource technology"
DOI: 10.1016/j.biortech.2018.01.148
Abstract: Samples of nitrogen-starved Parachlorella kessleri containing intact cells (IC), cells ground by bead milling (BM), and cells subjected to high-pressure cell disruption (HPD), together with their supernatants after centrifugation, were compared for granulometry and lipid… read more here.
Keywords: cell disruption; mechanical cell; parachlorella kessleri; disruption ... See more keywords
Cell disruption of Nannochloropsis sp. improves in vitro bioaccessibility of carotenoids and ω3-LC-PUFA
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Functional Foods"
DOI: 10.1016/j.jff.2019.103770
Abstract: Abstract The incorporation of microalgae into processed food products is considered a sustainable strategy to enrich food products in omega-3 long chain polyunsaturated fatty acids (ω3-LC-PUFA). However, the microstructural properties of the microalgae might influence… read more here.
Keywords: disruption nannochloropsis; cell disruption; bioaccessibility;
Rapid purification of bacteriophage endolysin TSPphg and its exogenous treatment could act as an alternative bacterial cell disruption method.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2018.03.014
Abstract: Bacteriophage endolysins have long been demonstrated to be effective enzybiotics, and have the potential value in the areas of food, agricultural, and industrial science. Traditionally, extraction of recombinant proteins from bacterium E. coli is achieved… read more here.
Keywords: disruption; bacterial cell; treatment; bacteriophage ... See more keywords
Machine learning modelling for the high-pressure homogenization-mediated disruption of recombinant E. coli
Sign Up to like & getrecommendations! Published in 2018 at "Process Biochemistry"
DOI: 10.1016/j.procbio.2018.05.001
Abstract: Abstract For French press mediated Escherichia coli cell disruption, the prediction capabilities of response surface method (RSM), multiple linear regression (MLR), multi-layer perceptron (MLP) and sequential minimal optimization (SMO) models are compared. The cell disruption… read more here.
Keywords: machine learning; disruption; coli; cell disruption ... See more keywords
Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids
Sign Up to like & getrecommendations! Published in 2021 at "Separation and Purification Technology"
DOI: 10.1016/j.seppur.2020.117759
Abstract: Abstract Recent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are… read more here.
Keywords: using imidazolium; talaromyces amestolkiae; cell disruption; cell ... See more keywords
Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-26241-7
Abstract: L-asparaginase, which catalyses the hydrolysis of L-asparagine to L-aspartate, has attracted the attention of researchers due to its expanded applications in medicine and the food industry. In this study, a novel thermostable L-asparaginase from Pyrococcus… read more here.
Keywords: high throughput; cell disruption; activity assays; asparaginase ... See more keywords