Deep Learning-Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard-to-Transfect Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202107795
Abstract: Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene… read more here.
Keywords: cell; electroporation; cell electroporation; manipulation ... See more keywords
Calibration of on-chip cell electroporation by a pseudo-volumetric uptake model.
Sign Up to like & getrecommendations! Published in 2017 at "Micron"
DOI: 10.1016/j.micron.2017.04.003
Abstract: Most conventional methods for assessing uptake of exogenous molecules and nanomaterials into cells use the projected two-dimensional (2D) area of uptake intensity into individual cells. However, since most cells have a three-dimensional (3D) spherical shape,… read more here.
Keywords: chip cell; cell electroporation; model; volumetric uptake ... See more keywords
Single-Cell Electroporation of Neurons.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot094904
Abstract: Single-cell electroporation allows the transfection of a small number of neurons in an organotypic culture with a single plasmid or a defined mixture of plasmids. Desired protein expression levels can vary depending on the experimental… read more here.
Keywords: single cell; electroporation neurons; electroporation; cell electroporation ... See more keywords