Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8567-8_9
Abstract: Over the past decade, ELISPOT has become a highly implemented mainstream assay in immunological research, immune monitoring, and vaccine development. Unique single cell resolution along with high throughput potential sets ELISPOT apart from flow cytometry,… read more here.
Keywords: cell fluorospot; color; multi color;
Multiplex analysis of antigen-specific memory B cells in humans using reversed B-cell FluoroSpot.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2019.112715
Abstract: Analysis of B-cell specificities at the single cell level provides important information on how the B-cell compartment responds when challenged by infection or vaccination. We recently developed a reversed B-cell FluoroSpot assay and showed that… read more here.
Keywords: analysis; antigen specific; cell fluorospot; antigen ... See more keywords