An improved digital polymerase chain reaction protocol to capture low‐copy KRAS mutations in plasma cell‐free DNA by resolving ‘subsampling’ issues
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12110
Abstract: Genetic alterations responsible for the initiation of cancer may serve as immediate biomarkers for early diagnosis. Plasma levels of cell‐free DNA (cfDNA) in patients with cancer are higher than those in healthy individuals; however, the… read more here.
Keywords: resolving subsampling; free dna; cell free; chain reaction ... See more keywords
EGFR transcription in non-small-cell lung cancer tumours can be revealed in ctDNA by cell-free chromatin immunoprecipitation (cfChIP).
Sign Up to like & getrecommendations! Published in 2021 at "Molecular oncology"
DOI: 10.1002/1878-0261.13093
Abstract: Determination of tumour-specific transcription based on liquid biopsies possesses a large diagnostic and prognostic potential in non-small cell lung cancer (NSCLC). Cell-free DNA (cfDNA) packed in nucleosomes mirrors the histone modification profiles present in the… read more here.
Keywords: transcription; cell free; cfchip; egfr l858r ... See more keywords
Clinical significance of circulating tumor cells and cell‐free DNA in pediatric rhabdomyosarcoma
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13197
Abstract: Liquid biopsy analysis represents a powerful and noninvasive tool to uncover biomarkers for disseminated disease assessment and longitudinal monitoring of patients. Herein, we explored the value of circulating and disseminated tumor cells (CTC and DTC,… read more here.
Keywords: cell free; free dna; tumor cells; pediatric rhabdomyosarcoma ... See more keywords
Cell‐free chromatin immunoprecipitation can determine tumor gene expression in lung cancer patients
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13394
Abstract: Cell‐free DNA (cfDNA) in blood plasma can be bound to nucleosomes that contain post‐translational modifications representing the epigenetic profile of the cell of origin. This includes histone H3 lysine 36 trimethylation (H3K36me3), a marker of… read more here.
Keywords: lung cancer; cell; cell free; cfchip ... See more keywords
mFast-SeqS-based aneuploidy score in circulating cell-free DNA is a prognostic biomarker in prostate cancer.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular oncology"
DOI: 10.1002/1878-0261.13449
Abstract: Multiple prognostic biomarkers, including circulating tumor cell (CTC) counts, exist in metastatic castration-resistant prostate cancer (mCRPC) patients, but none of them have been implemented into daily clinical care. The modified fast aneuploidy screening test-sequencing system… read more here.
Keywords: dna; cell free; mcrpc patients; aneuploidy score ... See more keywords
Engineered Microneedles for Interstitial Fluid Cell‐Free DNA Capture and Sensing Using Iontophoretic Dual‐Extraction Wearable Patch
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202000591
Abstract: Interstitial fluid (ISF), as an emerging source of biomarkers, is unmistakably significant for disease diagnosis. Microneedles (MNs) provide a minimally invasive approach for extracting the desired molecules from ISF. However, existing MNs are limited by… read more here.
Keywords: free dna; capture; interstitial fluid; cell free ... See more keywords
Artificial Vasa‐Vasorum Serves as an On‐Site Regenerative Promoter of Cell‐Free Vascular Grafting
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202315310
Abstract: The control paradigm of small vessel pathophysiology has changed to focus on the vascular out‐wall rather than the lumen‐intimal factors. As an emerging controller of the external wall, the microvasculature (“vasa vasorum”) provides interactional routes… read more here.
Keywords: artificial vasa; vasa vasorum; cell free; vascular ... See more keywords
Pathogenesis Activated “In Situ Disassembly‐Reassembly” Pattern Dual‐Scavenges Cell‐Free DNA/ROS for Hemorrhagic Stroke Management
Sign Up to like & getrecommendations! Published in 2025 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202424358
Abstract: Intracerebral hemorrhage (ICH) causes uncontrolled neuroinflammation/oxidative stress to aggravate secondary brain injury (SBI) for high mortality and disability but lacks effective pharmacotherapy in clinical practices. This represents an imperative need to discover potential targets and… read more here.
Keywords: free dna; cell free; disassembly reassembly; cfdna ... See more keywords
A Cell‐Free Silk Fibroin Biomaterial Strategy Promotes In Situ Cartilage Regeneration Via Programmed Releases of Bioactive Molecules
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202201588
Abstract: In situ tissue regeneration using cell‐free biofunctional scaffolds has been extensively studied as a promising alternative strategy to promote cartilage repair. In this study, a cartilage‐biomimetic silk fibroin (SF)‐based scaffold with controlled sequential release of… read more here.
Keywords: regeneration; cell free; silk fibroin; cartilage ... See more keywords
In Vitro Engineered ECM‐incorporated Hydrogels for Osteochondral Tissue Repair: A Cell‐Free Approach
Sign Up to like & getrecommendations! Published in 2025 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202402701
Abstract: Prevalence of osteoarthritis has been increasing in aging populations, which has necessitated the use of advanced biomedical treatments. These involve grafts or delivering drug molecules entrapped in scaffolds. However, such treatments often show suboptimal therapeutic… read more here.
Keywords: cell free; repair; engineered ecm; tissue repair ... See more keywords
Programmable Synthesis of Biobased Materials Using Cell‐Free Systems
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202203433
Abstract: Motivated by the intricate mechanisms underlying biomolecule syntheses in cells that chemistry is currently unable to mimic, researchers have harnessed biological systems for manufacturing novel materials. Cell‐free systems (CFSs) utilizing the bioactivity of transcriptional and… read more here.
Keywords: cell free; biobased materials; materials using; programmable synthesis ... See more keywords