An improved digital polymerase chain reaction protocol to capture low‐copy KRAS mutations in plasma cell‐free DNA by resolving ‘subsampling’ issues
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12110
Abstract: Genetic alterations responsible for the initiation of cancer may serve as immediate biomarkers for early diagnosis. Plasma levels of cell‐free DNA (cfDNA) in patients with cancer are higher than those in healthy individuals; however, the… read more here.
Keywords: resolving subsampling; free dna; cell free; chain reaction ... See more keywords
EGFR transcription in non-small-cell lung cancer tumours can be revealed in ctDNA by cell-free chromatin immunoprecipitation (cfChIP).
Sign Up to like & getrecommendations! Published in 2021 at "Molecular oncology"
DOI: 10.1002/1878-0261.13093
Abstract: Determination of tumour-specific transcription based on liquid biopsies possesses a large diagnostic and prognostic potential in non-small cell lung cancer (NSCLC). Cell-free DNA (cfDNA) packed in nucleosomes mirrors the histone modification profiles present in the… read more here.
Keywords: transcription; cell free; cfchip; egfr l858r ... See more keywords
Clinical significance of circulating tumor cells and cell‐free DNA in pediatric rhabdomyosarcoma
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13197
Abstract: Liquid biopsy analysis represents a powerful and noninvasive tool to uncover biomarkers for disseminated disease assessment and longitudinal monitoring of patients. Herein, we explored the value of circulating and disseminated tumor cells (CTC and DTC,… read more here.
Keywords: cell free; free dna; tumor cells; pediatric rhabdomyosarcoma ... See more keywords
Cell‐free chromatin immunoprecipitation can determine tumor gene expression in lung cancer patients
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13394
Abstract: Cell‐free DNA (cfDNA) in blood plasma can be bound to nucleosomes that contain post‐translational modifications representing the epigenetic profile of the cell of origin. This includes histone H3 lysine 36 trimethylation (H3K36me3), a marker of… read more here.
Keywords: lung cancer; cell; cell free; cfchip ... See more keywords
mFast-SeqS-based aneuploidy score in circulating cell-free DNA is a prognostic biomarker in prostate cancer.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular oncology"
DOI: 10.1002/1878-0261.13449
Abstract: Multiple prognostic biomarkers, including circulating tumor cell (CTC) counts, exist in metastatic castration-resistant prostate cancer (mCRPC) patients, but none of them have been implemented into daily clinical care. The modified fast aneuploidy screening test-sequencing system… read more here.
Keywords: dna; cell free; mcrpc patients; aneuploidy score ... See more keywords
Engineered Microneedles for Interstitial Fluid Cell‐Free DNA Capture and Sensing Using Iontophoretic Dual‐Extraction Wearable Patch
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202000591
Abstract: Interstitial fluid (ISF), as an emerging source of biomarkers, is unmistakably significant for disease diagnosis. Microneedles (MNs) provide a minimally invasive approach for extracting the desired molecules from ISF. However, existing MNs are limited by… read more here.
Keywords: free dna; capture; interstitial fluid; cell free ... See more keywords
A Cell‐Free Silk Fibroin Biomaterial Strategy Promotes In Situ Cartilage Regeneration Via Programmed Releases of Bioactive Molecules
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202201588
Abstract: In situ tissue regeneration using cell‐free biofunctional scaffolds has been extensively studied as a promising alternative strategy to promote cartilage repair. In this study, a cartilage‐biomimetic silk fibroin (SF)‐based scaffold with controlled sequential release of… read more here.
Keywords: regeneration; cell free; silk fibroin; cartilage ... See more keywords
Programmable Synthesis of Biobased Materials Using Cell‐Free Systems
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202203433
Abstract: Motivated by the intricate mechanisms underlying biomolecule syntheses in cells that chemistry is currently unable to mimic, researchers have harnessed biological systems for manufacturing novel materials. Cell‐free systems (CFSs) utilizing the bioactivity of transcriptional and… read more here.
Keywords: cell free; biobased materials; materials using; programmable synthesis ... See more keywords
A Cell‐free DNA Barcode‐Enabled Single‐Molecule Test for Noninvasive Prenatal Diagnosis of Monogenic Disorders: Application to β‐Thalassemia
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Science"
DOI: 10.1002/advs.201802332
Abstract: Abstract Noninvasive prenatal testing of common aneuploidies has become routine over the past decade, but testing of monogenic disorders remains a challenge in clinical implementation. Most recent studies have inherent limitations, such as complicated procedures,… read more here.
Keywords: molecule; monogenic disorders; barcode enabled; free dna ... See more keywords
A Cell‐Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.202001616
Abstract: Abstract Lanthipeptides have extensive therapeutic and industrial applications. However, because many are bactericidal, traditional in vivo platforms are limited in their capacity to discover and mass produce novel lanthipeptides as bacterial organisms are often critical… read more here.
Keywords: overproduction; vivo; cell free; platform ... See more keywords
Cell‐Free Protein Synthesis: A Cell‐Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo (Adv. Sci. 17/2020)
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.202070096
Abstract: In article number 2001616, Tiangang Liu and co-workers develop a cell-free protein synthesis (CFPS) platform that enables rapid discovery of novel lanthipeptides and guides lanthipeptide overproduction in vivo. This study extends the use of cell-free… read more here.
Keywords: cell free; protein synthesis; novel lanthipeptides; cell ... See more keywords