Robust preclinical evidence in somatic cell genome editing: a key driver of responsible and efficient therapeutic innovations.
Sign Up to like & getrecommendations! Published in 2021 at "Drug discovery today"
DOI: 10.1016/j.drudis.2021.06.007
Abstract: Somatic cell genome editing (SCGE) shows significant promising for therapeutic innovation. This study demonstrates that the majority of 46 preclinical SCGE studies discussed in reviews as particularly promising for clinical translation do not report on… read more here.
Keywords: genome editing; cell genome; research; somatic cell ... See more keywords
SiC-Seq: Single-cell genome sequencing at ultra high-throughput with microfluidic droplet barcoding
Sign Up to like & getrecommendations! Published in 2017 at "Nature biotechnology"
DOI: 10.1038/nbt.3880
Abstract: The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to… read more here.
Keywords: single cell; sic seq; ultra high; cell genome ... See more keywords
Regulation of single-cell genome organization into TADs and chromatin nanodomains
Sign Up to like & getrecommendations! Published in 2020 at "Nature genetics"
DOI: 10.1038/s41588-020-00716-8
Abstract: The genome folds into a hierarchy of three-dimensional (3D) structures within the nucleus. At the sub-megabase scale, chromosomes form topologically associating domains (TADs)1–4. However, how TADs fold in single cells remains elusive. Here, we revealed… read more here.
Keywords: single cell; regulation single; cell genome; cell ... See more keywords
Improving the efficiency of single-cell genome sequencing based on overlapping pooling strategy and CNV analysis
Sign Up to like & getrecommendations! Published in 2022 at "Royal Society Open Science"
DOI: 10.1098/rsos.211330
Abstract: Single-cell genome sequencing has become a useful tool in medicine and biology studies. However, an independent library is required for each cell in single-cell genome sequencing, so that the cost grows with the number of… read more here.
Keywords: cell genome; cell; cnv; genome sequencing ... See more keywords
HBV messing with the B-cell genome leads to DLBCL.
Sign Up to like & getrecommendations! Published in 2018 at "Blood"
DOI: 10.1182/blood-2018-05-847525
Abstract: In this issue of Blood, Ren et al report the results of a broad genomic and transcriptomic analysis of hepatitis B virus (HBV)–associated diffuse large B-cell lymphomas (DLBCLs) in Chinese patients, providing for the first… read more here.
Keywords: genome leads; cell genome; messing cell; cell ... See more keywords
Exploring strain diversity of dominant human skin bacterial species using single-cell genome sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.955404
Abstract: To understand the role of the skin commensal bacterial community in skin health and the spread of pathogens, it is crucial to identify genetic differences in the bacterial strains corresponding to human individuals. A culture-independent… read more here.
Keywords: cell genome; skin bacterial; single cell; skin ... See more keywords