Single-cell isoform RNA sequencing characterizes isoforms in thousands of cerebellar cells
Sign Up to like & getrecommendations! Published in 2018 at "Nature Biotechnology"
DOI: 10.1038/nbt.4259
Abstract: Full-length RNA sequencing (RNA-Seq) has been applied to bulk tissue, cell lines and sorted cells to characterize transcriptomes, but applying this technology to single cells has proven to be difficult, with less than ten single-cell… read more here.
Keywords: single cell; cell isoform; full length; rna sequencing ... See more keywords
Single-cell Iso-Sequencing enables rapid genome annotation for scRNAseq analysis
Sign Up to like & getrecommendations! Published in 2022 at "Genetics"
DOI: 10.1093/genetics/iyac017
Abstract: Abstract Single-cell RNA sequencing is a powerful technique that continues to expand across various biological applications. However, incomplete 3′-UTR annotations can impede single-cell analysis resulting in genes that are partially or completely uncounted. Performing single-cell… read more here.
Keywords: rna sequencing; single cell; cell; cell isoform ... See more keywords
Single-cell isoform analysis in human immune cells
Sign Up to like & getrecommendations! Published in 2022 at "Genome Biology"
DOI: 10.1186/s13059-022-02615-z
Abstract: High-throughput single-cell analysis today is facilitated by protocols like the 10X Genomics platform or Drop-Seq which generate cDNA pools in which the origin of a transcript is encoded at its 5′ or 3′ end. Here,… read more here.
Keywords: immune; single cell; isoform analysis; cell ... See more keywords