Functional role of glycosphingolipids in contact inhibition of growth in a human mammary epithelial cell line
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12709
Abstract: We have demonstrated previously the involvement of certain glycosphingolipids (GSLs) in ‘contact inhibition’ (dependent on cell‐to‐cell contact) of cell growth. Here, we examined the roles of specific GSLs in contact inhibition of the human epithelial… read more here.
Keywords: epithelial cell; cell line; inhibition; growth ... See more keywords
Facile synthesis of C6‐substituted benz[4,5]imidazo[1,2‐a]quinoxaline derivatives and their anticancer evaluation
Sign Up to like & getrecommendations! Published in 2021 at "Archiv der Pharmazie"
DOI: 10.1002/ardp.202000393
Abstract: Cancer remains a leading cause of death worldwide, resulting in continuous efforts to discover and develop highly efficacious anticancer drugs. High‐throughput screening of heterocyclic compound libraries is one of the promising approaches that provided several… read more here.
Keywords: quinoxaline derivatives; cell; cell line; cancer cell ... See more keywords
Upregulation of BAX and caspase‐3, as well as downregulation of Bcl‐2 during treatment with indeno[1,2‐b]quinoxalin derivatives, mediated apoptosis in human cancer cells
Sign Up to like & getrecommendations! Published in 2022 at "Archiv der Pharmazie"
DOI: 10.1002/ardp.202100454
Abstract: Cancer is the world's foremost cause of death. There are over 100 different forms of cancer. Cancers are frequently named after the organs or tissues in which they develop. As a part of our aim… read more here.
Keywords: cell; cell line; human cancer; downregulation bcl ... See more keywords
CRISPR/Cas12a‐mediated CHO genome engineering can be effectively integrated at multiple stages of the cell line generation process for bioproduction
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Journal"
DOI: 10.1002/biot.202000308
Abstract: Most biopharmaceuticals produced today are generated using Chinese hamster ovary (CHO) cells, therefore significant attention is focused on methods to improve CHO cell productivity and product quality. The discovery of gene‐editing tools, such as CRISPR/Cas9,… read more here.
Keywords: crispr cas12a; cho; cell; cell line ... See more keywords
A study of the THP‐1 cell line as the potential biologics production platform with the emphasis on serum‐free media substitution for economic expediency
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200154
Abstract: Cell cultures are frequently preferred in the industrial production of high value‐added biopharmaceuticals on a large scale. In the production of biopharmaceuticals, the selection of the appropriate cell line, the cell culture medium, and the… read more here.
Keywords: cell; cell line; thp; serum free ... See more keywords
Degradation of recombinant proteins by Chinese hamster ovary host cell proteases is prevented by matriptase‐1 knockout
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26731
Abstract: An increasing number of nonantibody format proteins are entering clinical development. However, one of the major hurdles for the production of nonantibody glycoproteins is host cell–related proteolytic degradation, which can drastically impact developability and timelines… read more here.
Keywords: degradation; cell line; chinese hamster; host ... See more keywords
A new nodavirus‐negative Trichoplusia ni cell line for baculovirus‐mediated protein production
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27494
Abstract: Cell lines derived from Trichoplusia ni (Tn) are widely used as hosts in the baculovirus‐insect cell system (BICS). One advantage of Tn cell lines is they can produce recombinant proteins at higher levels than cell… read more here.
Keywords: cell line; cell lines; nodavirus negative; trichoplusia ... See more keywords
A multiauxotrophic CHO cell line for the rapid isolation of producers of diverse or high levels of recombinant proteins
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28074
Abstract: Chinese Hamster Ovary (CHO) cells are widely used for the high‐level production of recombinant proteins. We created a multiauxotrophic mutant of CHO‐K1 cells, CHO8A, that is deficient in eight enzymatic steps in the purine/pyrimidine biosynthetic… read more here.
Keywords: cell; line rapid; recombinant proteins; cho cell ... See more keywords
Rapidly accelerated development of neutralizing COVID‐19 antibodies by reducing cell line and CMC development timelines
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28302
Abstract: Abstract Since the Coronavirus Disease 2019 (COVID‐19) outbreak, unconventional cell line development (CLD) strategies have been taken to enable development of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)‐neutralizing antibodies at expedited speed. We previously reported… read more here.
Keywords: cell; development timelines; development; cell line ... See more keywords
CRISPR‐interceded CHO cell line development approaches
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28329
Abstract: For industrial production of recombinant protein biopharmaceuticals, Chinese hamster ovary (CHO) cells represent the most widely adopted host cell system, owing to their capacity to produce high‐quality biologics with human‐like posttranslational modifications. As opposed to… read more here.
Keywords: line development; cell line; cell; cho cell ... See more keywords
Characterization of phenotypic and genotypic diversity in subclones derived from a clonal cell line
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2666
Abstract: Regulatory guidelines require the sponsors to provide assurance of clonality of the production cell line, and when such evidence is not available, additional studies are typically required to further ensure consistent long‐term manufacturing of the… read more here.
Keywords: cell line; derived clonal; diversity; phenotypic genotypic ... See more keywords