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   Articles with "cell lysate" as a keyword



Characterization of Mn3 O4 -MnO2 nanocomposites biosynthesized by cell lysate of Haloferax alexandrinus GUSF-1.

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recommendations! Published in 2023 at "Journal of basic microbiology"

DOI: 10.1002/jobm.202300023

Abstract: Manganese oxide nanocomposites attract huge attention in various biotechnological fields due to their extensive catalytic properties. This study reports an easy, rapid, and cost-effective method of using the cell lysate of haloarchaeon, Haloferax alexandrinus GUSF-1… read more here.

Keywords: mno2; haloferax alexandrinus; mn3 mno2; cell lysate ... See more keywords

The effects of biological crowders on fibrillization, structure, diffusion, and conformational dynamics of α‐synuclein

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recommendations! Published in 2024 at "Protein Science"

DOI: 10.1002/pro.4894

Abstract: α‐synuclein is an intrinsically disordered protein (IDP) whose aggregation in presynaptic neuronal cells is a pathological hallmark of Lewy body formation and Parkinson's disease. This aggregation process is likely affected by the crowded macromolecular cellular… read more here.

Keywords: structure; effects biological; cell lysate; cell ... See more keywords

Hydrogel composite of lanthanum and Halorubrum ejinoor sp. cell lysate as an adsorbing material.

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recommendations! Published in 2021 at "Biotechnology letters"

DOI: 10.1007/s10529-021-03132-y

Abstract: OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles. This study presents an simple method to prepare a… read more here.

Keywords: cell lysate; cell; hydrogel composite; adsorbing material ... See more keywords

Diffusion NMR and Rheology of a Model Polymer in Bacterial Cell Lysate Crowders.

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recommendations! Published in 2023 at "Biomacromolecules"

DOI: 10.1021/acs.biomac.2c01534

Abstract: The intracellular milieu is crowded and heterogeneous, and this can have profound consequences for biomolecule motions and biochemical kinetics. Macromolecular crowding has been traditionally studied in artificial crowders like Ficoll and dextran or globular proteins… read more here.

Keywords: rheology; bacterial cell; cell lysate; lysate ... See more keywords

Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors.

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recommendations! Published in 2017 at "ACS chemical biology"

DOI: 10.1021/acschembio.7b00543

Abstract: The deubiquitinases, or DUBs, are associated with various human diseases, including neurological disorders, cancer, and viral infection, making them excellent candidates for pharmacological intervention. Drug discovery campaigns against DUBs require enzymatic deubiquitination assays amenable for… read more here.

Keywords: small molecule; platform; molecule inhibitors; assay platform ... See more keywords

AuNis modified AlGaN/GaN HEMT biosensor for reliable detection of small Rho GTPases in Jurkat T cell lysate

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recommendations! Published in 2025 at "Scientific Reports"

DOI: 10.1038/s41598-025-18072-0

Abstract: A highly sensitive and stable biosensor was crucial for the early screening and treatment of diseases. As one of the most promising and powerful platforms, AlGaN/GaN HEMT biosensors have enormous potential owing to their distinctive… read more here.

Keywords: detection; gan hemt; cell lysate; algan gan ... See more keywords

CTLA-4 silencing in dendritic cells loaded with colorectal cancer cell lysate improves autologous T cell responses in vitro

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recommendations! Published in 2022 at "Frontiers in Immunology"

DOI: 10.3389/fimmu.2022.931316

Abstract: Dendritic cell (DC)-based immunotherapy has increased interest among anti-cancer immunotherapies. Nevertheless, the immunosuppressive mechanisms in the tumor milieu, e.g., inhibitory immune checkpoint molecules, have been implicated in diminishing the efficacy of DC-mediated anti-tumoral immune responses.… read more here.

Keywords: cell; ctla; cell responses; cell lysate ... See more keywords

Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli

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recommendations! Published in 2020 at "PeerJ"

DOI: 10.7717/peerj.9010

Abstract: The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that… read more here.

Keywords: cell lysate; oxidase; glucose oxidase; pastoris gs115 ... See more keywords