New horizons in developing cell lysis methods: A review
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28198
Abstract: Cell lysis is an essential step in many studies related to biology and medicine. Based on the scale and medium that cell lysis is carried out, there are three main types of the cell lysis:… read more here.
Keywords: cell lysis; new horizons; lysis; lysis methods ... See more keywords
Microfluidic electrical cell lysis for high-throughput and continuous production of cell-free varicella-zoster virus.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2021.06.006
Abstract: Varicella-zoster virus (VZV), the causative agent of varicella and herpes zoster, is highly cell-associated and spreads via cell-to-cell contact in tissue culture. The lack of cell-free VZV hampers studies on VZV biology as well as… read more here.
Keywords: electrical cell; cell lysis; vzv; cell free ... See more keywords
Electrical force-based continuous cell lysis and sample separation techniques for development of integrated microfluidic cell analysis system: A review
Sign Up to like & getrecommendations! Published in 2018 at "Microelectronic Engineering"
DOI: 10.1016/j.mee.2018.06.010
Abstract: Abstract Cell lysis and the separation of intracellular components are key steps in the analysis of intracellular components. Advances in micro-electro-mechanical system (MEMS) technologies have prompted a number of investigations into the potential applications of… read more here.
Keywords: sample separation; cell lysis; lysis sample; cell ... See more keywords
Diversity of Wadden Sea macrofauna and meiofauna communities highest in DNA from extractions preceded by cell lysis
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Sea Research"
DOI: 10.1016/j.seares.2019.101764
Abstract: Metabarcoding of genetic material in environmental samples has increasingly been employed as a means to assess biodiversity, also of marine benthic communities. Current protocols employed to extract DNA from benthic samples and subsequent bioinformatics pipelines… read more here.
Keywords: preceded cell; dna; wadden sea; sea ... See more keywords
Remodeling Bacillus amyloliquefaciens Cell Wall Rigidity to Reduce Cell Lysis and Increase the Yield of Heterologous Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c04454
Abstract: Bacillus amyloliquefaciens has great potential as a host for heterologous protein production, but its severe autolytic behavior has precluded its industrial application to date. Because d,l-endopeptidase activity-guided cell wall rigidity is considered essential for autolysis… read more here.
Keywords: wall rigidity; cell; cell wall; cell lysis ... See more keywords
Autoinduction AND Gate Inhibits Cell Lysis to Enhance Protein Production in Bacillus subtilis Controlled by Population Density and Cell Physiological State.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00624
Abstract: The extracellular protease-deficient strain Bacillus subtilis WB600 is commonly used as a chassis cell for the production of industrial proteins. However, B. subtilis WB600 exhibits an increased susceptibility to cell lysis and a reduction in biomass.… read more here.
Keywords: cell; cell lysis; subtilis wb600; production ... See more keywords
Development of simple and efficient Lab-on-a-Disc platforms for automated chemical cell lysis
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-67995-3
Abstract: Cell lysis is the most important first step for molecular biology and diagnostic testing. Recently, microfluidic systems have attracted considerable attention due to advantages associated with automation, integration and miniaturization, especially in resource-limited settings. In… read more here.
Keywords: cell lysis; chemical cell; lysis; cell ... See more keywords
Inertial microfluidics combined with selective cell lysis for high throughput separation of nucleated cells from whole blood
Sign Up to like & getrecommendations! Published in 2017 at "RSC Advances"
DOI: 10.1039/c7ra02992f
Abstract: The ability to rapidly analyze and extract information from peripheral blood cells has the potential of providing a wealth of new information about immune function and general health of the patient ... read more here.
Keywords: combined selective; cell lysis; inertial microfluidics; selective cell ... See more keywords
Investigating the effect of reagent parameters on the efficiency of cell lysis within droplets
Sign Up to like & getrecommendations! Published in 2020 at "Physics of Fluids"
DOI: 10.1063/5.0009840
Abstract: Cell lysis is an essential primary step in cell assays. In the process of cell lysis, the cell membrane is destroyed and the substances inside the cell are extracted. By utilizing a droplet-based microfluidic platform… read more here.
Keywords: droplet; cell lysis; effect; lysis ... See more keywords
Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2119286119
Abstract: Significance The extracellular concentration of adenosine triphosphate (ATP) reaches several hundred micromoles in the inflamed tissues or tumor environment. A high concentration of ATP activates P2X7, a purinergic receptor, and induces the formation of a… read more here.
Keywords: cell lysis; ptdser; p2x7; vps13a ... See more keywords
Effect of Cell Lysis (CLs) Products on Acidophilic Chemolithotrophic Microorganisms and the Role of Acidocella Species
Sign Up to like & getrecommendations! Published in 2017 at "Geomicrobiology Journal"
DOI: 10.1080/01490451.2017.1300203
Abstract: ABSTRACT Acidophilic chemolithotrophic microorganisms (CMs) are widely used for bioleaching of mineral resources. However, the growth of bacteria and their leaching activity are often inhibited (restricted) by organic components, e.g. lysates and exudates. The aims… read more here.
Keywords: acidophilic chemolithotrophic; cell lysis; chemolithotrophic microorganisms; effect ... See more keywords