Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity
Sign Up to like & getrecommendations! Published in 2020 at "Current Protocols in Immunology"
DOI: 10.1002/cpim.112
Abstract: Tight junctions form a selectively permeable barrier that limits paracellular flux across epithelial‐lined surfaces. Small molecules (less than ∼8 Å diameter) can traverse the junction via the size‐ and charge‐selective, high‐conductance pore pathway. In contrast,… read more here.
Keywords: epithelial cell; intestinal epithelial; cell monolayers; size ... See more keywords
Titanium dioxide nanoparticles translocate through differentiated Caco‐2 cell monolayers, without disrupting the barrier functionality or inducing genotoxic damage
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Applied Toxicology"
DOI: 10.1002/jat.3630
Abstract: The widespread use of titanium dioxide nanoparticles (TiO2NPs) in commercial food products makes intestinal cells a suitable target. Accordingly, we have used the human colon adenocarcinoma Caco‐2 cells to detect their potential harmful effects. Caco‐2… read more here.
Keywords: microscopy; cell monolayers; titanium dioxide; dioxide nanoparticles ... See more keywords
Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8600-2_15
Abstract: Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived… read more here.
Keywords: cell monolayers; human mouse; epithelial cell; mouse derived ... See more keywords
Acanthamoeba culbertsoni isolated from a clinical case with intraocular dissemination: Structure and in vitro analysis of the interaction with hamster cornea and MDCK epithelial cell monolayers.
Sign Up to like & getrecommendations! Published in 2017 at "Experimental parasitology"
DOI: 10.1016/j.exppara.2017.09.018
Abstract: Acanthamoeba culbertsoni trophozoites, previously isolated from a human keratitis case with severe intraocular damage, were maintained in axenic culture. Co-incubation of amoebae with MDCK cell monolayers demonstrated an apparent preference of the amoebae to introduce… read more here.
Keywords: acanthamoeba culbertsoni; cell monolayers; hamster; case ... See more keywords
Bridging microscopic cell dynamics to nematohydrodynamics of cell monolayers.
Sign Up to like & getrecommendations! Published in 2022 at "Soft matter"
DOI: 10.1039/d2sm00537a
Abstract: It is increasingly being realized that liquid-crystalline features can play an important role in the properties and dynamics of cell monolayers. Here, we present a cell-based model of cell layers, based on the phase-field formulation,… read more here.
Keywords: cell; cell monolayers; bridging microscopic; dynamics nematohydrodynamics ... See more keywords
Effects of hydrophobicity and molecular weight on the transport permeability of oligopeptides across Caco-2 cell monolayers.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of food biochemistry"
DOI: 10.1111/jfbc.13188
Abstract: The objective of this paper was to investigate the effects of hydrophobicity and molecular weight (MW) on the transepithelial transport permeability of oligopeptides across Caco-2 cell monolayers. Results showed that oligopeptides with different N-terminal amino… read more here.
Keywords: cell monolayers; transport; across caco; permeability ... See more keywords
Assessment of the DPP-IV inhibitory activity of a novel octapeptide derived from rapeseed using Caco-2 cell monolayers and molecular docking analysis.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of food biochemistry"
DOI: 10.1111/jfbc.13406
Abstract: The Octapeptide ELHQEEPL, which was identified from the rapeseed protein napin showed prominent Dipeptidyl peptidase-IV (DPP-IV) inhibitory activity. The objective of this study was to investigate the DPP-IV inhibitory activity and transepithelial transport of ELHQEEPL… read more here.
Keywords: cell monolayers; caco cell; activity; dpp inhibitory ... See more keywords
Improved recovery of cryopreserved cell monolayers with a hyaluronic acid surface treatment.
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DOI: 10.1116/6.0000613
Abstract: Cryopreservation is an essential part of tissue banking and effective cryopreservation methods are critical for the development of cost-effective cell therapy products. Cell sheets are an attractive subset of cell therapy types, and cryopreservation has… read more here.
Keywords: recovery; cell monolayers; surface; cryopreservation ... See more keywords
Drug-transporter mediated interactions between anthelminthic and antiretroviral drugs across the Caco-2 cell monolayers
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DOI: 10.1186/s40360-017-0129-6
Abstract: BackgroundDrug interactions between antiretroviral drugs (ARVs) and anthelminthic drugs, ivermectin (IVM) and praziquantel (PZQ) were assessed by investigating their permeation through the Caco-2 cell monolayers in a transwell. The impact of anthelminthics on the transport… read more here.
Keywords: cell monolayers; sqv; transport; drug ... See more keywords
Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.847861
Abstract: Gut barrier disruption is the initial pathogenesis of various diseases. We previously reported that dietary allicin improves tight junction proteins in the endoplasmic reticulum stressed jejunum. However, whether the allicin benefits the gut barrier within… read more here.
Keywords: barrier function; cell monolayers; barrier; induced barrier ... See more keywords
Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Nutrition"
DOI: 10.3389/fnut.2022.996607
Abstract: Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in… read more here.
Keywords: agaro oligosaccharides; caco cell; cell monolayers; permeation ... See more keywords