Probing the interaction of a quercetin bioconjugate with Bcl‐2 in living human cancer cells with in‐cell NMR spectroscopy
Sign Up to like & getrecommendations! Published in 2018 at "FEBS Letters"
DOI: 10.1002/1873-3468.13250
Abstract: In‐cell NMR spectroscopy has emerged as a powerful technique for monitoring biomolecular interactions at an atomic level inside intact cells. However, current methodologies are inadequate at charting intracellular interactions of nonlabeled proteins and require their… read more here.
Keywords: human cancer; living human; nmr spectroscopy; cell nmr ... See more keywords
In‐Cell NMR Spectroscopy of Nucleic Acids in Human Cells
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Nucleic Acid Chemistry"
DOI: 10.1002/cpnc.71
Abstract: In‐cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near‐to‐atomic resolution. In this… read more here.
Keywords: spectroscopy nucleic; nmr spectroscopy; cell nmr; spectroscopy ... See more keywords
Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-Cell NMR Spectroscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7386-6_20
Abstract: This paper describes three protocols for identifying interacting surfaces on 15N-labeled target proteins of known structure by using in-cell NMR spectroscopy. The first protocol describes how to identify protein quinary structure interaction surfaces in prokaryotes… read more here.
Keywords: nmr spectroscopy; cell nmr; spectroscopy; using cell ... See more keywords
Interaction proteomics by using in-cell NMR spectroscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2018.02.006
Abstract: A synopsis of in-cell NMR spectroscopic approaches to study interaction proteomics in prokaryotic and eukaryotic cells is presented. We describe the use of in-cell NMR spectroscopy to resolve high resolution protein structures, discuss methodologies for… read more here.
Keywords: cell nmr; interaction proteomics; nmr spectroscopy; spectroscopy ... See more keywords
In-cell NMR: From target structure and dynamics to drug screening.
Sign Up to like & getrecommendations! Published in 2022 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2022.102374
Abstract: The cellular environment can affect the structure and function of pharmacological targets and the interaction with potential drugs. Such complexity is often overlooked in the first steps of drug design, where compounds are screened and optimized… read more here.
Keywords: drug; drug screening; cell nmr; target ... See more keywords
Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c12086
Abstract: In-cell NMR spectroscopy is a powerful approach to study protein structure and function in the native cellular environment. It provides precious insights into the folding, maturation, interactions, and ligand binding of important pharmacological targets directly… read more here.
Keywords: 19f cell; spectroscopy; human cells; nmr spectroscopy ... See more keywords
In-cell NMR as a sensitive tool to monitor physiological condition of Escherichia coli
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-59076-2
Abstract: The in-cell NMR technique offers significant insights into the structure and function of heterologous proteins in the physiological intracellular environment at an atomic resolution. Escherichia coli (E. coli) is one of the most widely used… read more here.
Keywords: resolution; escherichia coli; cell nmr; cell ... See more keywords
Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc05576g
Abstract: We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the… read more here.
Keywords: interaction rna; human cells; rna aptamer; living human ... See more keywords
Applications of In-Cell NMR in Structural Biology and Drug Discovery
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20010139
Abstract: In-cell nuclear magnetic resonance (NMR) is a method to provide the structural information of a target at an atomic level under physiological conditions and a full view of the conformational changes of a protein caused… read more here.
Keywords: drug discovery; cell nmr; biology; applications cell ... See more keywords
In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20020378
Abstract: Nuclear magnetic resonance (NMR) spectroscopy enables the non-invasive observation of biochemical processes, in living cells, at comparably high spectral and temporal resolution. Preferably, means of increasing the detection limit of this powerful analytical method need… read more here.
Keywords: cell nmr; small molecule; protein small; molecule interactions ... See more keywords