Use of mitochondrial sequencing to detect gene doping in horses via gene editing and somatic cell nuclear transfer.
Sign Up to like & getrecommendations! Published in 2022 at "Drug testing and analysis"
DOI: 10.1002/dta.3267
Abstract: Gene editing and subsequent cloning techniques offer great potential not only in genetic disease correction in domestic animals, but also in livestock production by enhancement of desirable traits. The existence of the technology, however, leaves… read more here.
Keywords: mitochondrial sequencing; nuclear transfer; cell nuclear; somatic cell ... See more keywords
Molecular characterization and expression profile of an alternate proliferating cell nuclear antigen homolog PbPCNA2 in Plasmodium berghei
Sign Up to like & getrecommendations! Published in 2019 at "IUBMB Life"
DOI: 10.1002/iub.2036
Abstract: Proliferative cell nuclear antigen (PCNA) is the processivity factor for various DNA polymerases and it functions in response to DNA damage in eukaryotic system. Plasmodium falciparum contains two PCNAs, while PCNA1 has been attributed to… read more here.
Keywords: cell nuclear; pbpcna2; physiology; expression ... See more keywords
Sperm‐derived RNAs improve the efficiency of somatic cell nuclear transfer (SCNT) through promoting R‐loop formation
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23627
Abstract: Mammalian sperm and oocytes are haploid cells that carry parental genetic and epigenetic information for their progeny. The cytoplasm of oocytes is also capable of reprograming somatic cells to establish totipotency through somatic cell nuclear… read more here.
Keywords: sperm derived; somatic cell; cell nuclear; sperm ... See more keywords
Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9009-2_22
Abstract: Zebrafish (Danio rerio) is an established animal model to study developmental biology as well as a wide array of human diseases. Here we describe a protocol for somatic cell nuclear transfer (SCNT). This protocol can… read more here.
Keywords: somatic cell; cell nuclear; nuclear transfer; biology ... See more keywords
Episomal minicircles persist in periods of transcriptional inactivity and can be transmitted through somatic cell nuclear transfer into bovine embryos
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology Reports"
DOI: 10.1007/s11033-019-04624-x
Abstract: Episomal plasmids based on a scaffold/matrix attachment region (S/MAR) are extrachromosomal DNA entities that replicate once per cell cycle and are stably maintained in cells or tissue. We generated minicircles, episomal plasmids devoid of bacterial… read more here.
Keywords: somatic cell; cell nuclear; bovine; episomal minicircles ... See more keywords
Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.01.036
Abstract: (Cell 172, 881–887; February 8, 2018) In the above article, the raw data of RNA-sequencing, SNP, and STR were not initially uploaded into a public repository. These data have now been deposited and can be… read more here.
Keywords: monkeys somatic; somatic cell; cloning macaque; cell nuclear ... See more keywords
Myeloid cell nuclear differentiation antigen controls the pathogen-stimulated type I interferon cascade in human monocytes by transcriptional regulation of IRF7
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-021-27701-x
Abstract: Type I interferons (IFNs) are critical for anti-viral responses, and also drive autoimmunity when dysregulated. Upon viral sensing, monocytes elicit a sequential cascade of IFNβ and IFNα production involving feedback amplification, but how exactly this… read more here.
Keywords: ifn; cell nuclear; nuclear differentiation; interferon ... See more keywords
Factors Affecting Mouse Somatic Cell Nuclear Reprogramming by Rabbit Ooplasms.
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DOI: 10.1089/cell.2017.0021
Abstract: Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse… read more here.
Keywords: somatic cell; cell nuclear; embryos; iscnt ... See more keywords
Risk of pathogenic virus transmission by somatic cell nuclear transfer: implications for xenotransplantation
Sign Up to like & getrecommendations! Published in 2022 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioac120
Abstract: Abstract Using somatic cell nuclear transfer for the generation of cloned and transgenic animals bears the risk of transmission of viruses, either by the oocyte or by the introduced donor cell. There is evidence that… read more here.
Keywords: cell; nuclear transfer; transmission; somatic cell ... See more keywords
Down-Regulation of H3K4me3 by MM-102 Facilitates Epigenetic Reprogramming of Porcine Somatic Cell Nuclear Transfer Embryos
Sign Up to like & getrecommendations! Published in 2018 at "Cellular Physiology and Biochemistry"
DOI: 10.1159/000487579
Abstract: Background/Aims: Aberrantly high levels of H3K4me3, caused by incomplete epigenetic reprogramming, likely cause a low efficiency of somatic cell nuclear transfer (SCNT). Smal molecule inhibitors aimed at epigenetic modification can be used to improve porcine… read more here.
Keywords: somatic cell; cell nuclear; embryos; nuclear transfer ... See more keywords
Assessment of proliferating cell nuclear antigen (PCNA) expression at the invading front of oral squamous cell carcinoma
Sign Up to like & getrecommendations! Published in 2019 at "BMC Oral Health"
DOI: 10.1186/s12903-019-0928-9
Abstract: BackgroundAccurate prediction of the behaviour of oral squamous cell carcinoma (OSCC) is necessary to determine prognosis and provide appropriate treatment. Therefore, it is important to investigate potential prognostic markers to determine their predictive ability. Histological… read more here.
Keywords: cell; cell nuclear; proliferating cell; nuclear antigen ... See more keywords