Impact of Mutational Status on Intracellular Effects of Cell‐Permeable CaaX Peptides in Pancreatic Cancer Cells
Sign Up to like & getrecommendations! Published in 2025 at "Chembiochem"
DOI: 10.1002/cbic.202401076
Abstract: Prenyltransferases add a lipid group to the cysteine of a CaaX motif of proteins. This posttranslational modification enables proteins to attach to membranes where they are essential hubs for signaling, trafficking, and apoptosis. Recently, cell‐permeable… read more here.
Keywords: cancer cells; permeable caax; caax peptides; cell permeable ... See more keywords
Inhibition of sPLA2 enzyme activity by cell‐permeable antioxidant EUK‐8 and downregulation of p38, Akt, and p65 signals induced by sPLA2 in inflammatory mouse paw edema model
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.30366
Abstract: The arachidonic acid (AA) metabolic pathway, plays a vital role in the production of eicosanoids by the action of pro‐inflammatory secretory phospholipase A2 (PLA2). Release of eicosanoids is known to be involved in many inflammatory… read more here.
Keywords: cell; edema; permeable antioxidant; activity ... See more keywords
Designing Cell-Permeable Macrocyclic Peptides.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9504-2_3
Abstract: Peptides provide an attractive modality for targeting challenging drug targets such as intracellular protein-protein interactions. Unfortunately, peptides are generally impermeable to the cell membrane and inherently susceptible to proteolytic degradation in vivo. Macrocyclization of peptides… read more here.
Keywords: designing cell; cell permeable; macrocyclic peptides; cell penetrating ... See more keywords
Direct measurement of deubiquitinating enzyme activity in intact cells using a protease-resistant, cell-permeable, peptide-based reporter.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical engineering journal"
DOI: 10.1016/j.bej.2019.107320
Abstract: Deubiquitinating enzymes (DUBs) regulate the removal of the polyubiquitin chain from proteins targeted for degradation. Current approaches to quantify DUB activity are limited to test tube-based assays that incorporate enzymes or cell lysates, but not… read more here.
Keywords: cell permeable; activity; dub activity; peptide based ... See more keywords
Cell-Permeable Peptide Inhibitors of the p53-hDM2 Interaction via Foldamer Helix Mimicry and Bis-Thioether Stapling.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.4c01762
Abstract: Combining helical foldamers with α-peptides can produce α-helix mimetics with a reduced peptide character and enhanced resistance to proteolysis. Previously, we engineered a hybrid peptide-oligourea sequence replicating the N-terminal α-helical domain of p53 to achieve… read more here.
Keywords: permeable peptide; foldamer; helix; peptide ... See more keywords
Highly Potent Cell-Permeable and Impermeable NanoLuc Luciferase Inhibitors.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.6b01129
Abstract: Novel engineered NanoLuc (Nluc) luciferase being smaller, brighter, and superior to traditional firefly (Fluc) or Renilla (Rluc) provides a great opportunity for the development of numerous biological, biomedical, clinical, and food and environmental safety applications.… read more here.
Keywords: highly potent; cell permeable; potent cell; nluc ... See more keywords
Synthesis of Monofluorinated 7-Hydroxycoumarin-3-Carboxamides as Cell-Permeable Fluorescent Molecular Probes.
Sign Up to like & getrecommendations! Published in 2024 at "ACS medicinal chemistry letters"
DOI: 10.1021/acsmedchemlett.4c00085
Abstract: To facilitate studies of engagement of protein targets by small molecules in living cells, we synthesized fluorinated derivatives of the fluorophore 7-hydroxycoumarin-3-carboxylic acid (7OHCCA). Compared to the related difluorinated coumarin Pacific Blue (PB), amide derivatives… read more here.
Keywords: fluorescent molecular; synthesis monofluorinated; fluorescent; molecular probes ... See more keywords
Spatio-Temporal Autophagy Tracking with a Cell-Permeable, Water-Soluble, Peptide-Based, Autophagic Vesicle-Targeted Sensor.
Sign Up to like & getrecommendations! Published in 2021 at "ACS sensors"
DOI: 10.1021/acssensors.1c00191
Abstract: Autophagy is an essential cellular degradation process. Impaired autophagy has been linked to multiple disorders, including cancer and neurodegeneration. Tracking the autophagic flux in living cells will provide mechanistic insights into autophagy and will allow… read more here.
Keywords: cell permeable; water; autophagic vesicles; autophagy ... See more keywords
Cell-Permeable Bicyclic Peptidyl Inhibitors against NEMO-IκB Kinase Interaction Directly from a Combinatorial Library.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b06738
Abstract: Macrocyclic peptides are capable of binding to flat protein surfaces such as the interfaces of protein-protein interactions with antibody-like affinity and specificity, but generally lack cell permeability in order to access intracellular targets. In this… read more here.
Keywords: permeable bicyclic; cell permeable; kinase; combinatorial library ... See more keywords
Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01503-6
Abstract: Characterizing the long-term nanometer-scale interactions between lysosomes and mitochondria in live cells is essential for understanding their functions but remains challenging due to limitations of the existing fluorescent probes. Here, we develop cell-permeable organic fluorescent… read more here.
Keywords: resolution; cell permeable; microscopy; fluorescent ... See more keywords
A cell-permeable and triazole-forming fluorescent probe for glycoconjugate imaging in live cells.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c6cc08805h
Abstract: A new fluorescence-forming probe, coumOCT, designed by fusing cyclooctyne with a coumarin fluorophore was successfully used for the imaging of azido-glycoconjugates in living HeLa cells. This probe is cell-permeable and generates fluorescence after triazole formation,… read more here.
Keywords: forming fluorescent; cell permeable; probe; glycoconjugate ... See more keywords