Inhibition of sPLA2 enzyme activity by cell‐permeable antioxidant EUK‐8 and downregulation of p38, Akt, and p65 signals induced by sPLA2 in inflammatory mouse paw edema model
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.30366
Abstract: The arachidonic acid (AA) metabolic pathway, plays a vital role in the production of eicosanoids by the action of pro‐inflammatory secretory phospholipase A2 (PLA2). Release of eicosanoids is known to be involved in many inflammatory… read more here.
Keywords: cell; edema; permeable antioxidant; activity ... See more keywords
Designing Cell-Permeable Macrocyclic Peptides.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9504-2_3
Abstract: Peptides provide an attractive modality for targeting challenging drug targets such as intracellular protein-protein interactions. Unfortunately, peptides are generally impermeable to the cell membrane and inherently susceptible to proteolytic degradation in vivo. Macrocyclization of peptides… read more here.
Keywords: designing cell; cell permeable; macrocyclic peptides; cell penetrating ... See more keywords
Direct measurement of deubiquitinating enzyme activity in intact cells using a protease-resistant, cell-permeable, peptide-based reporter.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical engineering journal"
DOI: 10.1016/j.bej.2019.107320
Abstract: Deubiquitinating enzymes (DUBs) regulate the removal of the polyubiquitin chain from proteins targeted for degradation. Current approaches to quantify DUB activity are limited to test tube-based assays that incorporate enzymes or cell lysates, but not… read more here.
Keywords: cell permeable; activity; dub activity; peptide based ... See more keywords
Highly Potent Cell-Permeable and Impermeable NanoLuc Luciferase Inhibitors.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.6b01129
Abstract: Novel engineered NanoLuc (Nluc) luciferase being smaller, brighter, and superior to traditional firefly (Fluc) or Renilla (Rluc) provides a great opportunity for the development of numerous biological, biomedical, clinical, and food and environmental safety applications.… read more here.
Keywords: highly potent; cell permeable; potent cell; nluc ... See more keywords
Spatio-Temporal Autophagy Tracking with a Cell-Permeable, Water-Soluble, Peptide-Based, Autophagic Vesicle-Targeted Sensor.
Sign Up to like & getrecommendations! Published in 2021 at "ACS sensors"
DOI: 10.1021/acssensors.1c00191
Abstract: Autophagy is an essential cellular degradation process. Impaired autophagy has been linked to multiple disorders, including cancer and neurodegeneration. Tracking the autophagic flux in living cells will provide mechanistic insights into autophagy and will allow… read more here.
Keywords: cell permeable; water; autophagic vesicles; autophagy ... See more keywords
Cell-Permeable Bicyclic Peptidyl Inhibitors against NEMO-IκB Kinase Interaction Directly from a Combinatorial Library.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b06738
Abstract: Macrocyclic peptides are capable of binding to flat protein surfaces such as the interfaces of protein-protein interactions with antibody-like affinity and specificity, but generally lack cell permeability in order to access intracellular targets. In this… read more here.
Keywords: permeable bicyclic; cell permeable; kinase; combinatorial library ... See more keywords
Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01503-6
Abstract: Characterizing the long-term nanometer-scale interactions between lysosomes and mitochondria in live cells is essential for understanding their functions but remains challenging due to limitations of the existing fluorescent probes. Here, we develop cell-permeable organic fluorescent… read more here.
Keywords: resolution; cell permeable; microscopy; fluorescent ... See more keywords
A cell-permeable and triazole-forming fluorescent probe for glycoconjugate imaging in live cells.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c6cc08805h
Abstract: A new fluorescence-forming probe, coumOCT, designed by fusing cyclooctyne with a coumarin fluorophore was successfully used for the imaging of azido-glycoconjugates in living HeLa cells. This probe is cell-permeable and generates fluorescence after triazole formation,… read more here.
Keywords: forming fluorescent; cell permeable; probe; glycoconjugate ... See more keywords
Targeting NF-κB by the Cell-Permeable NEMO-Binding Domain Peptide Improves Albuminuria and Renal Lesions in an Experimental Model of Type 2 Diabetic Nephropathy
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21124225
Abstract: Diabetic nephropathy (DN) is a multifactorial disease characterized by hyperglycemia and close interaction of hemodynamic, metabolic and inflammatory factors. Nuclear factor-κB (NF-κB) is a principal matchmaker linking hyperglycemia and inflammation. The present work investigates the… read more here.
Keywords: peptide; binding domain; cell permeable; nemo binding ... See more keywords
Cell-Permeable Succinate Rescues Mitochondrial Respiration in Cellular Models of Amiodarone Toxicity
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms222111786
Abstract: Amiodarone is a potent antiarrhythmic drug and displays substantial liver toxicity in humans. It has previously been demonstrated that amiodarone and its metabolite (desethylamiodarone, DEA) can inhibit mitochondrial function, particularly complexes I (CI) and II… read more here.
Keywords: mitochondrial respiration; cell permeable; respiration; permeable succinate ... See more keywords