The Intriguing Landscape of Single‐Cell Protein Analysis
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202105932
Abstract: Profiling protein expression at single‐cell resolution is essential for fundamental biological research (such as cell differentiation and tumor microenvironmental examination) and clinical precision medicine where only a limited number of primary cells are permitted. With… read more here.
Keywords: cell protein; cell; single cell; protein analysis ... See more keywords
Experience with host cell protein impurities in biopharmaceuticals
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2640
Abstract: In the 40‐year history of biopharmaceuticals, there have been a few cases where the final products contained residual host cell protein (HCP) impurities at levels high enough to be of concern. This article summarizes the… read more here.
Keywords: host cell; cell protein; hcp impurities; experience ... See more keywords
Fabrication of glycated yeast cell protein via Maillard reaction for delivery of curcumin: Improved environmental stability, antioxidant activity, and bioaccessibility.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.12413
Abstract: BACKGROUND The application of curcumin (CUR) in food industry is limited by its instability, hydrophobicity and low bioavailability. Yeast cell protein (YCP) is a by-product of spent brewer's yeast, which has the potential to deliver… read more here.
Keywords: reaction; bioaccessibility; yeast cell; cell protein ... See more keywords
Kupffer Cell Protein Isolation and Detection by Western Blot.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0704-6_4
Abstract: Kupffer cells are the majority of tissue macrophages present in the body and are actively involved in the regulation of liver homeostasis as well as in the progression of liver diseases. Here we describe a… read more here.
Keywords: kupffer; protein isolation; kupffer cell; cell ... See more keywords
Single-Cell Protein Assays: A Review.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7717-8_17
Abstract: Quantification of single-cell proteomics provides key insights in the field of cellular heterogeneity. This chapter discusses the emerging techniques that are being used to measure the protein copy numbers at the single-cell level, which includes… read more here.
Keywords: single cell; protein assays; assays review; cell ... See more keywords
Co-production of lipid, exopolysaccharide and single-cell protein by Sporidiobolus pararoseus under ammonia nitrogen-limited conditions
Sign Up to like & getrecommendations! Published in 2020 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-020-02335-3
Abstract: With the rapid depletion of crude resources, microorganism lipids have started attracting increasing attention because of their renewable qualities. However, their production is limited by high costs. In this study, we aimed to reduce the… read more here.
Keywords: single cell; production; ammonia nitrogen; sporidiobolus pararoseus ... See more keywords
CHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2019.02.006
Abstract: Abstract Several studies on the impact of cell culture parameters on the profile of host cell protein (HCP) impurities have been carried out in shake flasks. Herein, we explore how transferable the findings and conclusions… read more here.
Keywords: cell protein; host cell; cell; shake flasks ... See more keywords
Urban biowaste valorization by coupling anaerobic digestion and single cell protein production.
Sign Up to like & getrecommendations! Published in 2019 at "Bioresource technology"
DOI: 10.1016/j.biortech.2019.121743
Abstract: Resource efficient and novel practices to produce proteinaceous food and feed sources can partially alleviate the protein scarcity problem. The conversion of low-value waste streams into single cell protein (SCP) seems a potent solution. This… read more here.
Keywords: protein; single cell; production; urban biowaste ... See more keywords
Bioconversion of wastewater to single cell protein by methanotrophic bacteria.
Sign Up to like & getrecommendations! Published in 2020 at "Bioresource technology"
DOI: 10.1016/j.biortech.2020.124351
Abstract: Single cell protein (SCP) provides an alternative protein source to partially replace the conventional agricultural resources and support the increased nutritional needs. Inexpensive feeding source is one of the key limiting factors for the expansion… read more here.
Keywords: protein; single cell; source; methanotrophic bacteria ... See more keywords
Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2018.01.004
Abstract: Chinese hamster ovary (CHO) cells are the most prevalent host organism for production of recombinant therapeutic proteins, including monoclonal antibodies (mAbs). Regulatory guidance mandates control of the host cell protein (HCP) concentration in the production… read more here.
Keywords: applications proteomic; host cell; cell protein; host ... See more keywords
Almond hulls waste valorization towards sustainable agricultural development: Production of pectin, phenolics, pullulan, and single cell protein.
Sign Up to like & getrecommendations! Published in 2022 at "Waste management"
DOI: 10.1016/j.wasman.2022.01.007
Abstract: This research aimed to valorize almond hulls based on a zero-waste strategy towards sustainable agricultural developments for the recovery and production of valuable compounds. For this purpose, the potential to produce four products, including pectin… read more here.
Keywords: almond hulls; towards sustainable; sustainable agricultural; single cell ... See more keywords