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1
Published in 2023 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202200060
Abstract: Multiple control strategies, including a downstream purification process with well‐controlled parameters and a comprehensive release or characterization for intermediates or drug substances, were implemented to mitigate the potential risk of host cell proteins (HCPs) in…
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Keywords:
host cell;
cell proteins;
control;
cfb ... See more keywords
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2
Published in 2023 at "Proteomics"
DOI: 10.1002/pmic.202300172
Abstract: Therapeutic monoclonal antibodies (mAb) production relies on multiple purification steps before release as a drug product (DP). A few host cell proteins (HCPs) may co-purify with the mAb. Their monitoring is crucial due to the…
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Keywords:
quantification;
mass spectrometry;
level;
spectrometry ... See more keywords
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2
Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6972-2_16
Abstract: Host cell protein content during bioprocessing of biotherapeutic proteins generated from cultured Chinese hamster ovary (CHO) cells is typically measured using immunological and gel-based methods. Estimation of HCP concentration is usually undertaken using Enzyme-Linked ImmunoSorbent…
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Keywords:
host cell;
characterization host;
cell proteins;
cho ... See more keywords
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1
Published in 2018 at "Current Opinion in Chemical Engineering"
DOI: 10.1016/j.coche.2018.08.001
Abstract: Monoclonal antibodies hold a steadfast lead in the ever-expanding biologics marketplace and have revolutionized the treatment of a wide variety of illnesses. The prominence of mAbs as therapeutic agents brought with it the need for…
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Keywords:
host cell;
mab bioprocessing;
cell proteins;
cell ... See more keywords
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0
Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2018.03.002
Abstract: Recombinantly expressed biopharmaceutical proteins often undergo a series of purification steps with the aim of removing contaminating material. Depending on the application of the protein, there are various requirements for the degree of purity, but…
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Keywords:
protein;
biopharmaceutical protein;
host cell;
spectral libraries ... See more keywords
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2
Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00020
Abstract: Certain host cell proteins (HCPs) in biotherapeutic drugs may be detrimental to drug product quality even when they are present at the subppm level. Therefore, an analytical method that can reliably quantify trace amounts of…
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Keywords:
quantification;
ppm;
cell proteins;
drug products ... See more keywords
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Published in 2020 at "IEEE Journal of Translational Engineering in Health and Medicine"
DOI: 10.1109/jtehm.2020.2963894
Abstract: Objective: investigation of the extra-low-frequency (ELF) stimulation effect on blood-cell proteins, that causes variation in its electrostatic-state. A hypothesis that this results in the conformational change in the blood-cell proteins which could enhance immune activity…
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Keywords:
medicine;
cell proteins;
activity;
blood cell ... See more keywords
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Published in 2023 at "Analytical biochemistry"
DOI: 10.2139/ssrn.4229856
Abstract: Liquid chromatography coupled to mass spectrometry (LC-MS) is widely used for host cell proteins (HCP) identification in antibody drug development because of its sensitivity, selectivity, and adaptability. However, LC-MS based identification of HCP in biotherapeutics…
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Keywords:
standard spiking;
hcp;
growth hormone;
cell proteins ... See more keywords
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2
Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23179994
Abstract: Beta-herpesvirus infection completely reorganizes the membrane system of the cell. This system is maintained by the spatiotemporal arrangement of more than 3000 cellular proteins that continuously adapt the configuration of membrane organelles according to cellular…
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Keywords:
cell;
beta;
cell signatures;
cell proteins ... See more keywords